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Literature summary extracted from

  • Inouye, S.; Sasaki, S.
    Imidazole-assisted catalysis of luminescence reaction in blue fluorescent protein from the photoprotein aequorin (2007), Biochem. Biophys. Res. Commun., 354, 650-655 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.12.24 imidazole luminescence activity of BFP-aq 1s stimulated 1.5- to 2fold by imidazole at concentrations of 30-300 mM. Imidazole has no effect on the binding affinity of coelenterazine and may act as a catalytic base Aequorea victoria
1.13.12.24 additional information 1H-pyrazole, 2-methylimidazole, 4-methylimidazole, histamine, L-histidine, urocanic acid, and 5-amino-4-imidazole carboxamide do not act as stimulators at concentrations of 30 and 150 mM Aequorea victoria

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.24 NaCl half-maximal inhibition was at 600 mM Aequorea victoria

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.24 Aequorea victoria P02592
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.24 additional information poor substrates: n-coelenterazine, bis-coelenterazine, methoxy-coelenterazine, e-coelenterazine Aequorea victoria ?
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+
-
 Aequorea victoria [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + cp-coelenterazine + O2 + 3 Ca2+
-
 Aequorea victoria [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + f-coelenterazine + O2 + 3 Ca2+
-
 Aequorea victoria [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + h-coelenterazine + O2 + 3 Ca2+
-
 Aequorea victoria [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + hcp-coelenterazine + O2 + 3 Ca2+
-
 Aequorea victoria [excited state blue fluorescent protein] + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.24 BFP-aq
-
 Aequorea victoria
1.13.12.24 blue fluorescent protein
-
 Aequorea victoria