EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.26.5 | ribonuclease P protein components 1, 2, 3 and 4 are expressed in Escherichia coli cells, and the resulting proteins Ph1481p, Ph1601p, Ph1771p, and Ph1877p are purified to apparent homogeneity in a set of column chromatographies. RNase P reconstituted of four proteins with the in vitro transcripted Pyrococcus horikoshii RNase P RNA exhibits enzymatic properties like those of native enzyme | Pyrococcus horikoshii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.5 | pre-tRNA + H2O | Pyrococcus horikoshii | - |
mature tRNA + 5'-terminal oligonucleotide | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.26.5 | Pyrococcus horikoshii | O59425 and O59150 and O59543 and O59248 and P62009 | O59425: ribonuclease P protein component 1 (PhoRpp29), O59150: ribonuclease P protein component 2 (PhoPop5), O59543: ribonuclease P protein component 3 (PhoRpp30), O59248: ribonuclease P protein component 4 (RPP21), 50S ribosomal protein L7Ae (PhoRpp38). RNase P consists of a catalytic RNase P RNA (PhopRNA) and five protein cofactors designated PhoPop5, PhoRpp21, PhoRpp29, PhoRpp30, and PhoRpp38 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.26.5 | ribonuclease P protein components 1, 2, 3 and 4 are expressed in Escherichia coli cells, and the resulting proteins Ph1481p, Ph1601p, Ph1771p, and Ph1877p are purified to apparent homogeneity in a set of column chromatographies. RNase P reconstituted of four proteins with the in vitro transcripted Pyrococcus horikoshii RNase P RNA exhibits enzymatic properties like those of native enzyme | Pyrococcus horikoshii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.5 | pre-tRNA + H2O | - |
Pyrococcus horikoshii | mature tRNA + 5'-terminal oligonucleotide | - |
? | |
3.1.26.5 | pre-tRNA(Tyr) + H2O | - |
Pyrococcus horikoshii | mature tRNA(Tyr) + 5'-terminal oligonucleotide | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.26.5 | tetramer | ribonuclease P protein component 1 (PH1771), ribonuclease P protein component 2 (PH1481), ribonuclease P protein component 3 (PH1877), ribonuclease P protein component 4 (PH1601). Three proteins Ph1481p, Ph1601p, and Ph1771p, and RNase P RNA are minimal components for the RNase P activity. However, addition of the fourth protein Ph1877p strongly stimulated enzymatic activity, indicating that all four proteins and RNase P RNA are essential for optimal RNase P activity | Pyrococcus horikoshii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.26.5 | RNase P | - |
Pyrococcus horikoshii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.26.5 | 37 | - |
assay at | Pyrococcus horikoshii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.26.5 | 7.6 | - |
assay at | Pyrococcus horikoshii |