EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.59 | expression in Pseudomonas fluorescens and Pseudomonas chlororaphis | Streptomyces albogriseolus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.19.59 | 0.11 | - |
L-tryptophan | pH 7.2, 30°C | Streptomyces albogriseolus | |
1.14.19.59 | 0.31 | - |
D-tryptophan | pH 7.2, 30°C | Streptomyces albogriseolus | |
1.14.19.59 | 1.32 | - |
bromide | pH 7.2, 30°C | Streptomyces albogriseolus | |
1.14.19.59 | 2.98 | - |
chloride | pH 7.2, 30°C | Streptomyces albogriseolus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.59 | Streptomyces albogriseolus | A1E280 | - |
- |
1.14.19.59 | Streptomyces albogriseolus MJ286-76F7 | A1E280 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.59 | D-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces albogriseolus | 6-chloro-D-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.59 | D-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces albogriseolus MJ286-76F7 | 6-chloro-D-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.59 | L-tryptophan + FADH2 + bromide + O2 + H+ | - |
Streptomyces albogriseolus | 6-bromo-L-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.59 | L-tryptophan + FADH2 + bromide + O2 + H+ | - |
Streptomyces albogriseolus MJ286-76F7 | 6-bromo-L-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.59 | L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces albogriseolus | 6-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.59 | L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces albogriseolus MJ286-76F7 | 6-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.59 | additional information | enzyme catalyses the regioselective chlorination and bromination of L- and D-tryptophan. In vitro activity of the purified enzyme can only be shown in a two-component enzyme system consisting of the halogenase, a flavin reductase, NADH, FAD and halide ions | Streptomyces albogriseolus | ? | - |
? | |
1.14.19.59 | additional information | enzyme catalyses the regioselective chlorination and bromination of L- and D-tryptophan. In vitro activity of the purified enzyme can only be shown in a two-component enzyme system consisting of the halogenase, a flavin reductase, NADH, FAD and halide ions | Streptomyces albogriseolus MJ286-76F7 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.19.59 | ? | x * 60000, SDS-PAGE | Streptomyces albogriseolus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.19.59 | 0.047 | - |
L-tryptophan | pH 7.2, 30°C | Streptomyces albogriseolus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.19.59 | physiological function | transformation of the pyrrolnitrin producer Pseudomonas chlororaphis ACN with a plasmid containing the tryptophan 6-halogenase gene leads to the formation of the aminopyrrolnitrin derivative 3-(2'-amino-4'-chlorophenyl)pyrrole | Streptomyces albogriseolus |