BRENDA - Enzyme Database

Physiological role of the F420-non-reducing hydrogenase (MvH) from Methanothermobacter marburgensis

Stojanowic, A.; Mander, G.; Duin, E.; Hedderich, R.; Arch. Microbiol. 180, 194-203 (2003)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.98.5
H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
Methanothermobacter marburgensis
-
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
-
?
1.8.98.5
H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
Methanothermobacter marburgensis DSM 2133
-
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.98.5
Methanothermobacter marburgensis
-
-
-
1.8.98.5
Methanothermobacter marburgensis DSM 2133
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.98.5
DEAE-Sepharose and Q-Sepharose column chromatography, and Superdex 200 gel filtration
Methanothermobacter marburgensis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.5
H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
744179
Methanothermobacter marburgensis
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
-
-
?
1.8.98.5
H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
744179
Methanothermobacter marburgensis DSM 2133
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
-
-
?
1.8.98.5
H2 + oxidized methyl viologen + CoM-S-S-CoB
-
744179
Methanothermobacter marburgensis
reduced methyl viologen + CoB + CoM + H+
-
-
-
?
1.8.98.5
H2 + oxidized methyl viologen + CoM-S-S-CoB
-
744179
Methanothermobacter marburgensis DSM 2133
reduced methyl viologen + CoB + CoM + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.8.98.5
?
x * 51000 + x * 41000 + x * 17000, SDS-PAGE
Methanothermobacter marburgensis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.98.5
[2Fe-2S]-center
-
Methanothermobacter marburgensis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.98.5
[2Fe-2S]-center
-
Methanothermobacter marburgensis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.98.5
H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
Methanothermobacter marburgensis
-
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
-
?
1.8.98.5
H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
Methanothermobacter marburgensis DSM 2133
-
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.98.5
DEAE-Sepharose and Q-Sepharose column chromatography, and Superdex 200 gel filtration
Methanothermobacter marburgensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.5
H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
744179
Methanothermobacter marburgensis
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
-
-
?
1.8.98.5
H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
744179
Methanothermobacter marburgensis DSM 2133
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
-
-
?
1.8.98.5
H2 + oxidized methyl viologen + CoM-S-S-CoB
-
744179
Methanothermobacter marburgensis
reduced methyl viologen + CoB + CoM + H+
-
-
-
?
1.8.98.5
H2 + oxidized methyl viologen + CoM-S-S-CoB
-
744179
Methanothermobacter marburgensis DSM 2133
reduced methyl viologen + CoB + CoM + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.98.5
?
x * 51000 + x * 41000 + x * 17000, SDS-PAGE
Methanothermobacter marburgensis