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Literature summary extracted from

  • Guo, H.F.; Cho, E.J.; Devkota, A.K.; Chen, Y.; Russell, W.; Phillips, G.N.; Yamauchi, M.; Dalby, K.N.; Kurie, J.M.
    A scalable lysyl hydroxylase 2 expression system and luciferase-based enzymatic activity assay (2017), Arch. Biochem. Biophys., 618, 45-51 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.4 ascorbate required for activity Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.4 gene PLOD2, large-scale transfection with N-terminal His8 and human growth hormone (hGH) tags transfection with polyethylenimine and recombinant expression of wild-type enzyme, N-terminally truncated enzyme mutant, and of inactive mutant D689A in engineered CHO cells and secretion to the cell culture medium Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.4 D689A inactive mutant Homo sapiens
1.14.11.4 additional information generation of an N-terminally truncated enzyme mutant comprising residues 33-758 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.4 2-oxoglutarate competitive substrate inhibition Homo sapiens
1.14.11.4 L-alpha-hydroxyglutarate the enzyme activity is inhibited by L-alpha-hydroxyglutarate but not its enantiomer D-alpha-hydroxyglutarate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.4 additional information
-
 additional information steady-state kinetics of LH2 Homo sapiens
1.14.11.4 0.011
-
 2-oxoglutarate pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
1.14.11.4 0.169
-
 peptide (IKG)3 pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.4 Fe2+ dependent on Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.4 [procollagen]-L-lysine + 2-oxoglutarate + O2 Homo sapiens
-
 [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.4 Homo sapiens O00469
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.11.4 glycoprotein identification of LH2 glycosylation by nanoLC MS/MS analysis Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.4 additional information an enzyme activity assay for detection of succinate is evaluated: the succinate-Glo assay, employing a luciferase-based luminescence system, is superior from the standpoint of stability and signal-to-noise ratio. Luciferase activity is detected over a wide range of concentrations of LH2, and the luminescence signal was proportional to the concentration of LH2. Method development and evaluation, overview Homo sapiens ?
-
 ?
1.14.11.4 peptide (IKG)3 + 2-oxoglutarate + O2
-
 Homo sapiens ?
-
 ?
1.14.11.4 [procollagen]-L-lysine + 2-oxoglutarate + O2
-
 Homo sapiens [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.4 LH2
-
 Homo sapiens
1.14.11.4 lysyl hydroxylase 2
-
 Homo sapiens
1.14.11.4 PLOD2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.4 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.4 0.183
-
 2-oxoglutarate pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
1.14.11.4 0.2
-
 peptide (IKG)3 pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.4 7.4
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.11.4 evolution the enzyme belongs to the 2-oxoglutarate/Fe2+-dependent oxygenase family Homo sapiens
1.14.11.4 physiological function hydroxylysine aldehyde-derived collagen cross-links (HLCCs) accumulate in fibrotic tissues and certain types of cancer and are thought to drive the progression of these diseases. HLCC formation is initiated by lysyl hydroxylase 2 (LH2), an Fe(II) and 2-oxoglutarate-dependent oxygenase that hydroxylates telopeptidyl lysine residues on collagen Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.11.4 1.18
-
 peptide (IKG)3 pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
1.14.11.4 16.6
-
 2-oxoglutarate pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens