EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.6 | gene lysS, recombinant expression of His-tagged enzyme in Escherichia coli | Myxococcus xanthus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.6 | ADP | the addition of ADP inhibits the diadenosine tetraphosphate synthesis by Ap4A synthase activity of LysS. Ap4A production decreases by 22% and 52% at 1 mM and 5 mM ADP, respectively. In the presence of ADP, Ap3A is not synthesized by LysS during 30 min of incubation. ADP may act as a competitive inhibitor of LysS in the first step reaction, i.e. lysyl-AMP formation from lysine and ATP | Myxococcus xanthus | |
6.1.1.6 | Zn2+ | inhibits enzyme activity in the presence of Mn2+ | Myxococcus xanthus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.6 | Mg2+ | required | Myxococcus xanthus | |
6.1.1.6 | Mn2+ | the enzyme requires Mn2+ for the synthesis of diadenosine tetraphosphate (Ap4A) | Myxococcus xanthus | |
6.1.1.6 | additional information | Zn2+ is not required for diadenosine tetraphosphate (Ap4A) synthesis by LysS | Myxococcus xanthus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.1.1.6 | 68000 | - |
recombinant His-tagged enzyme, gel filtration | Myxococcus xanthus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.6 | ATP + L-lysine + tRNALys | Myxococcus xanthus | - |
AMP + diphosphate + L-lysyl-tRNALys | - |
? | |
6.1.1.6 | ATP + L-lysine + tRNALys | Myxococcus xanthus DK 1622 | - |
AMP + diphosphate + L-lysyl-tRNALys | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.6 | Myxococcus xanthus | Q1D380 | - |
- |
6.1.1.6 | Myxococcus xanthus DK 1622 | Q1D380 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.6 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, dialysis, EDTA treatment, and again dialysis | Myxococcus xanthus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.6 | ATP + L-lysine + tRNALys | - |
Myxococcus xanthus | AMP + diphosphate + L-lysyl-tRNALys | - |
? | |
6.1.1.6 | ATP + L-lysine + tRNALys | - |
Myxococcus xanthus DK 1622 | AMP + diphosphate + L-lysyl-tRNALys | - |
? | |
6.1.1.6 | additional information | lysyl-tRNA synthetases efficiently produce diadenosine tetraphosphate (Ap4A) from lysyl-AMP with ATP in the absence of tRNA. When incubated with ATP, Mn2+, lysine, and inorganic diphosphatase, LysS first produces Ap4A and ADP, then converts Ap4A to diadenosine triphosphate (Ap3A), and finally converts Ap3A to ADP, the end product of the reaction. Recombinant LysS retains Ap4A synthase activity without lysine addition. When incubated with Ap4A (minus pyrophosphatase), LysS converts Ap4A mainly ATP and AMP, or ADP in the presence or absence of lysine, respectively. LysS converts Ap4A to yield ATP and AMP when Mn2+ and lysine are present, formation of ATP is higher than that of AMP. LysS hydrolyzes Ap4A symmetrically yielding ADP | Myxococcus xanthus | ? | - |
? | |
6.1.1.6 | additional information | lysyl-tRNA synthetases efficiently produce diadenosine tetraphosphate (Ap4A) from lysyl-AMP with ATP in the absence of tRNA. When incubated with ATP, Mn2+, lysine, and inorganic diphosphatase, LysS first produces Ap4A and ADP, then converts Ap4A to diadenosine triphosphate (Ap3A), and finally converts Ap3A to ADP, the end product of the reaction. Recombinant LysS retains Ap4A synthase activity without lysine addition. When incubated with Ap4A (minus pyrophosphatase), LysS converts Ap4A mainly ATP and AMP, or ADP in the presence or absence of lysine, respectively. LysS converts Ap4A to yield ATP and AMP when Mn2+ and lysine are present, formation of ATP is higher than that of AMP. LysS hydrolyzes Ap4A symmetrically yielding ADP | Myxococcus xanthus DK 1622 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.1.1.6 | monomer | 1 x 58000, about, recombinant His-tagged enzyme, sequence calculation, 1 * 60000, recombinant His-tagged enzyme, SDS-PAGE | Myxococcus xanthus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.6 | Ap4A synthase | - |
Myxococcus xanthus |
6.1.1.6 | class II lysyl-tRNA synthetase | - |
Myxococcus xanthus |
6.1.1.6 | lysS | - |
Myxococcus xanthus |
6.1.1.6 | MXAN_4731 | - |
Myxococcus xanthus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.6 | 50 | - |
- |
Myxococcus xanthus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.6 | 30 | 60 | the relative activity of LysS at 30-60°C is 49%, 77%, or 45%, respectively, compared to the activity at the optimal temperature of 50°C | Myxococcus xanthus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.6 | 8 | 9 | - |
Myxococcus xanthus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.6 | 7 | 9 | optimal activity at pH 8.0-9.0, 80% of maximal activity at pH 7.0 | Myxococcus xanthus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.6 | ATP | - |
Myxococcus xanthus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
6.1.1.6 | evolution | the lysyl-tRNA synthetase of Mycobacterium smegmatis belongs to the class II amino acid tRNA synthetases | Myxococcus xanthus |
6.1.1.6 | physiological function | activity of most class II aminoacyl-tRNA synthetases absolutely requires the presence of added cognate amino acids | Myxococcus xanthus |