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Literature summary extracted from

  • Schlangen, K.; Miosic, S.; Halbwirth, H.
    Allelic variants from Dahlia variabilis encode with functional differences in chalcone 3-hydroxylase activity (2010), Arch. Biochem. Biophys., 494, 40-45 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.B18
-
 Dahlia pinnata

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.B18 V425A complete loss of the ability th hydroxylate isoliquiritigenin in position 3 Dahlia pinnata
1.14.14.82 V425A mutation in allele 2, complete loss of the ability th hydroxylate the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase Dahlia pinnata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.B18 0.0039
-
 isoliquiritigenin pH 7.5, 30°C Dahlia pinnata
1.14.14.82 0.0005
-
 naringenin allele 2, pH 7.5, 30°C Dahlia pinnata
1.14.14.82 0.0009
-
 naringenin allele 1, pH 7.5, 30°C Dahlia pinnata
1.14.14.82 0.0013
-
 dihydrokaempferol allele 2, pH 7.5, 30°C Dahlia pinnata
1.14.14.82 0.0035
-
 dihydrokaempferol allele 1, pH 7.5, 30°C Dahlia pinnata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 Dahlia pinnata
-
 dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 additional information Dahlia pinnata allele 2 additionally catalyzes the hydroxylation of the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase ?
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2 Dahlia pinnata
-
 eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.B18 Dahlia pinnata D3IWE6 D3IWE6 i.e. allele 2, enzyme additionally catalyzes the reaction of EC 1.14.14.82
-
1.14.14.82 Dahlia pinnata D3IWE5 and D3IWE6 D3IWE5 i.e. allele 1, D3IWE6 i.e. allele 2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.B18 isoliquiritigenin + 2-oxoglutarate + O2 hydroxylation in position 3, i.e. reaction of chalcone 3-hydroxylase Dahlia pinnata butein + succinate + CO2
-
 ?
1.14.11.B18 naringenin chalcone + 2-oxoglutarate + O2
-
 Dahlia pinnata eriodictyol chalcone
-
 ?
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Dahlia pinnata dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 additional information allele 2 additionally catalyzes the hydroxylation of the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase Dahlia pinnata ?
-
 ?
1.14.14.82 additional information allele 2 addtionally catalyzes the hydroxylation of the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase Dahlia pinnata ?
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Dahlia pinnata eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.B18 Cyp75B
-
 Dahlia pinnata
1.14.14.82 Cyp75B
-
 Dahlia pinnata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.B18 7.5
-
-
 Dahlia pinnata
1.14.14.82 7.3
-
 both allele 1 and 2 Dahlia pinnata