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Literature summary extracted from

  • Nishio, T.; Patel, A.; Wang, Y.; Lau, P.C.
    Biotransformations catalyzed by cloned p-cymene monooxygenase from Pseudomonas putida F1 (2001), Appl. Microbiol. Biotechnol., 55, 321-325 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.25 expression in Escherichia coli Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.25 Pseudomonas putida O33456 and O33457 O33456 i.e. subunit CymAa, O33457 i.e. subunit CymAb
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.25 4-(methylthio)toluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-(methylthio)benzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.25 4-chlorostyrene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-chlorostyrene oxide + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.25 4-ethyltoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-ethylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.25 4-fluorotoluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-fluorobenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.25 p-cymene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida 4-isopropylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.25 styrene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Pseudomonas putida styrene oxide + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.25 Ferredoxin
-
 Pseudomonas putida