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Literature summary extracted from

  • Frommhagen, M.; Westphal, A.; Hilgers, R.; Koetsier, M.; Hinz, S.; Visser, J.; Gruppen, H.; van Berkel, W.; Kabel, M.
    Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases (2018), Appl. Microbiol. Biotechnol., 102, 1281-1295 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.99.54 analysis development of a beta-glucosidase-assisted method to quantify the release of C1-oxidized glucooligosaccharides from cellulose Thermothelomyces thermophilus
1.14.99.54 analysis development of a beta-glucosidase-assisted method to quantify the release of C1-oxidized glucooligosaccharides from cellulose Thermothelomyces heterothallicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.54 Thermothelomyces heterothallicus A0A218MJF1
-
-
1.14.99.54 Thermothelomyces thermophilus A0A1C9CXI1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.54 regenerated amorphous cellulose + 3-methylcatechol + O2
-
 Thermothelomyces thermophilus 3-methyl-o-benzoquinone + H2O
-
 ?
1.14.99.54 regenerated amorphous cellulose + 3-methylcatechol + O2
-
 Thermothelomyces heterothallicus ? + 3-methyl-o-benzoquinone + H2O
-
 ?
1.14.99.54 regenerated amorphous cellulose + ascorbic acid + O2
-
 Thermothelomyces heterothallicus ? + dehydroascorbic acid + H2O
-
 ?
1.14.99.54 regenerated amorphous cellulose + ascorbic acid + O2
-
 Thermothelomyces thermophilus dehydroascorbic acid + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.54 LPMO9B
-
 Thermothelomyces thermophilus
1.14.99.54 LPMO9D
-
 Thermothelomyces heterothallicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.54 37
-
 assay at Thermothelomyces thermophilus
1.14.99.54 37
-
 assay at Thermothelomyces heterothallicus
1.14.99.54 40
-
 optimum in presence of 3-methylcatechol Thermothelomyces heterothallicus
1.14.99.54 40
-
 optimum in presence of ascorbic acid Thermothelomyces thermophilus
1.14.99.54 50
-
 optimum in presence of ascorbic acid Thermothelomyces heterothallicus
1.14.99.54 50 60 optimum in presence of 3-methylcatechol Thermothelomyces thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.54 5
-
 assay at Thermothelomyces thermophilus
1.14.99.54 5
-
 assay at Thermothelomyces heterothallicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.99.54 3 5 hardly any catalytic performance at a low pH Thermothelomyces heterothallicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.99.54 6
-
 inactivation within the first 6 h at pH values above 6 Thermothelomyces thermophilus