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Literature summary extracted from

  • Baek, S.C.; Ho, T.H.; Lee, H.W.; Jung, W.K.; Gang, H.S.; Kang, L.W.; Kim, H.
    Improvement of enzyme activity of beta-1,3-1,4-glucanase from Paenibacillus sp. X4 by error-prone PCR and structural insights of mutated residues (2017), Appl. Microbiol. Biotechnol., 101, 4073-4083 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.73 hanging drop vapor diffusion method, using 7.5% (v/v) 2-methyl-2,4-pentanediol, 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, and 0.1 M Tris (base)/Bicine, pH 8.5 Paenibacillus sp. X4
3.2.1.73 to 1.8 A resolution Paenibacillus sp. X4

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.73 C17R/Q87T/D152G/Y307H/V330A/N344D 2.1fold increase in specific activity Paenibacillus sp. X4
3.2.1.73 C17R/Q87T/D152G/Y307H/V330A/N344D the mutant shows 200.8% activity at pH 5.5 and 50°C compared to the wild type enzyme Paenibacillus sp. X4
3.2.1.73 E149D/V370E the mutant shows 59.9% activity at pH 5.5 and 50°C compared to the wild type enzyme Paenibacillus sp. X4
3.2.1.73 K12R/F30L/N114D/N252D/T348S/N380S the mutant shows 62.1% activity at pH 5.5 and 50°C compared to the wild type enzyme Paenibacillus sp. X4
3.2.1.73 additional information truncated mutants, which comprise 360, 286, and 215 amino acid residues instead of the 409 residues of the wild type, show complete loss of enzymatic activity Paenibacillus sp. X4
3.2.1.73 N197D/M322V 1.2fold increase in specific activity Paenibacillus sp. X4
3.2.1.73 N197D/M322V the mutant shows 144.7% activity at pH 5.5 and 50°C compared to the wild type enzyme Paenibacillus sp. X4
3.2.1.73 N4S the mutant shows 75.3% activity at pH 5.5 and 50°C compared to the wild type enzyme Paenibacillus sp. X4
3.2.1.73 S11R/T210S the mutant shows 231.3% activity at pH 5.5 and 50°C compared to the wild type enzyme Paenibacillus sp. X4
3.2.1.73 Y389F/ K395R the mutant shows 81% activity at pH 5.5 and 50°C compared to the wild type enzyme Paenibacillus sp. X4

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.73 Cu2+
-
 Paenibacillus sp. X4
3.2.1.73 Fe2+
-
 Paenibacillus sp. X4
3.2.1.73 Mn2+
-
 Paenibacillus sp. X4

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.73 additional information
-
 barley beta-D-glucan Km value of wild-type 13.5 mg/ml, mutant C17R/Q87T/D152G/Y307H/V330A/N344D 13.3 mg/ml, respectively, pH 5.0, 60°C Paenibacillus sp. X4
3.2.1.73 additional information
-
 barley beta-D-glucan Km value of wild-type 19.7 mg/ml, mutant C17R/Q87T/D152G/Y307H/V330A/N344D 16.3 mg/ml, respectively, pH 5.0, 40°C Paenibacillus sp. X4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.73 Ca2+
-
 Paenibacillus sp. X4
3.2.1.73 additional information not influenced by Na+, K+, Mg2+, or Zn2+ Paenibacillus sp. X4

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Paenibacillus sp. X4 A0A088BCU2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 Hi-Trap Q column chromatography and CHT-II column chromatography Paenibacillus sp. X4

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 607
-
 wild-type, pH 5.0, 40°C Paenibacillus sp. X4
3.2.1.73 724
-
 mutant N197D/M322V, pH 5.0, 40°C Paenibacillus sp. X4
3.2.1.73 1282
-
 mutant C17R/Q87T/D152G/Y307H/V330A/N344D, pH 5.0, 40°C Paenibacillus sp. X4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 barley beta-D-glucan + H2O
-
 Paenibacillus sp. X4 ?
-
 ?
3.2.1.73 barley beta-glucan + H2O 100% activity Paenibacillus sp. X4 ?
-
 ?
3.2.1.73 carboxymethyl cellulose + H2O 22.4% activity compared to barley beta-glucan Paenibacillus sp. X4 ?
-
 ?
3.2.1.73 laminarin + H2O 0.12% activity compared to barley beta-glucan Paenibacillus sp. X4 ?
-
 ?
3.2.1.73 lichenan + H2O 136.4% activity compared to barley beta-glucan Paenibacillus sp. X4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 ? x * 40000, SDS-PAGE Paenibacillus sp. X4

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 beta-1,3-1,4-glucanase
-
 Paenibacillus sp. X4
3.2.1.73 BGlc8H
-
 Paenibacillus sp. X4
3.2.1.73 Lic8H
-
 Paenibacillus sp. X4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 40
-
-
 Paenibacillus sp. X4

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 60
-
 after 30 min and 1 h at 60°C, the enzyme retains about 60% and 35% of activity, respectively Paenibacillus sp. X4

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.73 235
-
 Barley beta-glucan wild type enzyme, at pH 5.0 and 60°C Paenibacillus sp. X4
3.2.1.73 710
-
 Barley beta-glucan mutant enzyme C17R/Q87T/D152G/Y307H/V330A/N344D, at pH 5.0 and 60°C Paenibacillus sp. X4
3.2.1.73 2067
-
 Barley beta-glucan wild type enzyme, at pH 5.0 and 40°C Paenibacillus sp. X4
3.2.1.73 3628
-
 Barley beta-glucan mutant enzyme C17R/Q87T/D152G/Y307H/V330A/N344D, at pH 5.0 and 40°C Paenibacillus sp. X4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 5
-
-
 Paenibacillus sp. X4