BRENDA - Enzyme Database show

Production of 10R-hydroxy unsaturated fatty acids from hempseed oil hydrolyzate by recombinant Escherichia coli cells expressing PpoC from Aspergillus nidulans

Han, J.E.; Seo, M.J.; Shin, K.C.; Oh, D.K.; Appl. Microbiol. Biotechnol. 100, 7933-7944 (2016)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.13.11.62
synthesis
under the optimized conditions, the biotechnological production of (8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12-trienoate and (8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate by enzyme PpoC from Aspergillus nidulans strain ATCC 10074 from linoleic acid, alpha-linolenic acid, and hempseed oil hydrolyzate as substrates is achieved, method optimization
Aspergillus nidulans
Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.62
functional recombinant expression of His-tagged enzyme in Escherichia coli strain ER2566
Aspergillus nidulans
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.13.11.62
additional information
under the optimized conditions, the biotechnological production of (8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12-trienoate and (8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate by enzyme PpoC from Aspergillus nidulans strain ATCC 10074 from linoleic acid, alpha-linolenic acid, and hempseed oil hydrolyzate as substrates is achieved, method optimization, optimization of concentrations of cells and substrate, pH and temperature, for the production of 10R-hydroxy unsaturated fatty acids, overview. Recombinant enzyme PpoC in Escherichia coli cells is more stable than the purified recombinant PpoC
Aspergillus nidulans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.62
alpha-linolenate + O2
Aspergillus nidulans
-
(8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
?
1.13.11.62
alpha-linolenate + O2
Aspergillus nidulans ATCC 10074
-
(8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
?
1.13.11.62
linoleate + O2
Aspergillus nidulans
-
(8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
1.13.11.62
linoleate + O2
Aspergillus nidulans ATCC 10074
-
(8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
1.13.11.62
additional information
Aspergillus nidulans
the enzyme produces both (8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate and (8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate from hempseed oil hydrolyzate
?
-
-
-
1.13.11.62
additional information
Aspergillus nidulans ATCC 10074
the enzyme produces both (8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate and (8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate from hempseed oil hydrolyzate
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.62
Aspergillus nidulans
-
-
-
1.13.11.62
Aspergillus nidulans ATCC 10074
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.13.11.62
recombinant His-tagged enzyme from Escherichia coli strain ER2566 by nickel affinity chromatography and ultafiltration
Aspergillus nidulans
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.62
0.065
-
purified recombinant enzyme, substrate oleate, pH 8.0, 30°C
Aspergillus nidulans
1.13.11.62
0.108
-
purified recombinant enzyme, substrate palmitoleate, pH 8.0, 30°C
Aspergillus nidulans
1.13.11.62
0.485
-
purified recombinant enzyme, substrate alpha-linolenate, pH 8.0, 30°C
Aspergillus nidulans
1.13.11.62
0.761
-
purified recombinant enzyme, substrate linoleate, pH 8.0, 30°C
Aspergillus nidulans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.62
alpha-linolenate + O2
-
744123
Aspergillus nidulans
(8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
1.13.11.62
alpha-linolenate + O2
moderate activity
744123
Aspergillus nidulans
(8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
1.13.11.62
alpha-linolenate + O2
-
744123
Aspergillus nidulans ATCC 10074
(8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
1.13.11.62
alpha-linolenate + O2
moderate activity
744123
Aspergillus nidulans ATCC 10074
(8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
1.13.11.62
linoleate + O2
-
744123
Aspergillus nidulans
(8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
1.13.11.62
linoleate + O2
best substrate
744123
Aspergillus nidulans
(8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
1.13.11.62
linoleate + O2
-
744123
Aspergillus nidulans ATCC 10074
(8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
1.13.11.62
additional information
the enzyme produces both (8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate and (8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate from hempseed oil hydrolyzate
744123
Aspergillus nidulans
?
-
-
-
-
1.13.11.62
additional information
assay method optimization for the recombinant enzyme exprssed in Escherichia coli, overview
744123
Aspergillus nidulans
?
-
-
-
-
1.13.11.62
additional information
the enzyme produces both (8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate and (8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate from hempseed oil hydrolyzate
744123
Aspergillus nidulans ATCC 10074
?
-
-
-
-
1.13.11.62
additional information
assay method optimization for the recombinant enzyme exprssed in Escherichia coli, overview
744123
Aspergillus nidulans ATCC 10074
?
-
-
-
-
1.13.11.62
oleate + O2
low activity
744123
Aspergillus nidulans
8-hydroperoxy-9(Z)-octadecenoic acid
-
-
-
?
1.13.11.62
palmitoleate + O2
low activity
744123
Aspergillus nidulans
8-hydroperoxy-9(Z)-hexadecenoic acid
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.11.62
30
-
recombinant enzyme
Aspergillus nidulans
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.13.11.62
20
40
over 70% of maximal activity within this range
Aspergillus nidulans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.13.11.62
30
40
half-lives of recombinant PpoC in Escherichia coli cells at 30°C, 35°C, and 40°C are 530, 315, and 97 min, respectively, while those of purified recombinant enzyme are 41, 29, and 18 min, respectively. The half-lives of the recombinant enzyme in Escherichia coli cells are 12.9, 10.9, and 5.4fold higher at 30°C, 35°C, and 40°C, respectively, than those of the purified recombinant enzyme, indicating that thermal stability of recombinant PpoC in expressing cells is higher than that of the purified recombinant PpoC
Aspergillus nidulans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.62
8
-
recombinant enzyme
Aspergillus nidulans
Application (protein specific)
EC Number
Application
Commentary
Organism
1.13.11.62
synthesis
under the optimized conditions, the biotechnological production of (8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12-trienoate and (8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate by enzyme PpoC from Aspergillus nidulans strain ATCC 10074 from linoleic acid, alpha-linolenic acid, and hempseed oil hydrolyzate as substrates is achieved, method optimization
Aspergillus nidulans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.62
functional recombinant expression of His-tagged enzyme in Escherichia coli strain ER2566
Aspergillus nidulans
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.13.11.62
additional information
under the optimized conditions, the biotechnological production of (8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12-trienoate and (8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate by enzyme PpoC from Aspergillus nidulans strain ATCC 10074 from linoleic acid, alpha-linolenic acid, and hempseed oil hydrolyzate as substrates is achieved, method optimization, optimization of concentrations of cells and substrate, pH and temperature, for the production of 10R-hydroxy unsaturated fatty acids, overview. Recombinant enzyme PpoC in Escherichia coli cells is more stable than the purified recombinant PpoC
Aspergillus nidulans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.62
alpha-linolenate + O2
Aspergillus nidulans
-
(8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
?
1.13.11.62
alpha-linolenate + O2
Aspergillus nidulans ATCC 10074
-
(8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
?
1.13.11.62
linoleate + O2
Aspergillus nidulans
-
(8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
1.13.11.62
linoleate + O2
Aspergillus nidulans ATCC 10074
-
(8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
1.13.11.62
additional information
Aspergillus nidulans
the enzyme produces both (8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate and (8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate from hempseed oil hydrolyzate
?
-
-
-
1.13.11.62
additional information
Aspergillus nidulans ATCC 10074
the enzyme produces both (8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate and (8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate from hempseed oil hydrolyzate
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.62
recombinant His-tagged enzyme from Escherichia coli strain ER2566 by nickel affinity chromatography and ultafiltration
Aspergillus nidulans
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.62
0.065
-
purified recombinant enzyme, substrate oleate, pH 8.0, 30°C
Aspergillus nidulans
1.13.11.62
0.108
-
purified recombinant enzyme, substrate palmitoleate, pH 8.0, 30°C
Aspergillus nidulans
1.13.11.62
0.485
-
purified recombinant enzyme, substrate alpha-linolenate, pH 8.0, 30°C
Aspergillus nidulans
1.13.11.62
0.761
-
purified recombinant enzyme, substrate linoleate, pH 8.0, 30°C
Aspergillus nidulans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.62
alpha-linolenate + O2
-
744123
Aspergillus nidulans
(8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
1.13.11.62
alpha-linolenate + O2
moderate activity
744123
Aspergillus nidulans
(8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
1.13.11.62
alpha-linolenate + O2
-
744123
Aspergillus nidulans ATCC 10074
(8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
1.13.11.62
alpha-linolenate + O2
moderate activity
744123
Aspergillus nidulans ATCC 10074
(8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
1.13.11.62
linoleate + O2
-
744123
Aspergillus nidulans
(8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
1.13.11.62
linoleate + O2
best substrate
744123
Aspergillus nidulans
(8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
1.13.11.62
linoleate + O2
-
744123
Aspergillus nidulans ATCC 10074
(8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
1.13.11.62
additional information
the enzyme produces both (8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate and (8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate from hempseed oil hydrolyzate
744123
Aspergillus nidulans
?
-
-
-
-
1.13.11.62
additional information
assay method optimization for the recombinant enzyme exprssed in Escherichia coli, overview
744123
Aspergillus nidulans
?
-
-
-
-
1.13.11.62
additional information
the enzyme produces both (8E,10R,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate and (8E,10R,12Z)-10-hydroperoxyoctadeca-8,12-dienoate from hempseed oil hydrolyzate
744123
Aspergillus nidulans ATCC 10074
?
-
-
-
-
1.13.11.62
additional information
assay method optimization for the recombinant enzyme exprssed in Escherichia coli, overview
744123
Aspergillus nidulans ATCC 10074
?
-
-
-
-
1.13.11.62
oleate + O2
low activity
744123
Aspergillus nidulans
8-hydroperoxy-9(Z)-octadecenoic acid
-
-
-
?
1.13.11.62
palmitoleate + O2
low activity
744123
Aspergillus nidulans
8-hydroperoxy-9(Z)-hexadecenoic acid
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.11.62
30
-
recombinant enzyme
Aspergillus nidulans
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.13.11.62
20
40
over 70% of maximal activity within this range
Aspergillus nidulans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.13.11.62
30
40
half-lives of recombinant PpoC in Escherichia coli cells at 30°C, 35°C, and 40°C are 530, 315, and 97 min, respectively, while those of purified recombinant enzyme are 41, 29, and 18 min, respectively. The half-lives of the recombinant enzyme in Escherichia coli cells are 12.9, 10.9, and 5.4fold higher at 30°C, 35°C, and 40°C, respectively, than those of the purified recombinant enzyme, indicating that thermal stability of recombinant PpoC in expressing cells is higher than that of the purified recombinant PpoC
Aspergillus nidulans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.62
8
-
recombinant enzyme
Aspergillus nidulans
General Information
EC Number
General Information
Commentary
Organism
1.13.11.62
evolution
Psi-producing oxygenases (Ppo enzymes) are classified as three enzyme types: PpoA (5,8-diol synthase), PpoB (suggested as 8,11-diol synthase), and PpoC (10R-dioxygenase)
Aspergillus nidulans
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.13.11.62
evolution
Psi-producing oxygenases (Ppo enzymes) are classified as three enzyme types: PpoA (5,8-diol synthase), PpoB (suggested as 8,11-diol synthase), and PpoC (10R-dioxygenase)
Aspergillus nidulans