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Literature summary extracted from

  • Uraji, M.; Tamura, H.; Mizohata, E.; Arima, J.; Wan, K.; Ogawa, K.; Inoue, T.; Hatanaka, T.
    Loop of Streptomyces feruloyl esterase plays an important role in the enzymes catalyzing the release of ferulic acid from biomass (2018), Appl. Environ. Microbiol., 84, e02300 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 D214A mutation within catalytic triad, complete loss of activity Streptomyces cinnamoneus
3.1.1.73 H268A mutation within catalytic triad, complete loss of activity Streptomyces cinnamoneus
3.1.1.73 additional information construction of chimeric proteins by RIBS in vivo DNA shuffling of the feruloyl esterase genes of strains Streptomyces cinnamoneus NBRC 12852 and Streptomyces cinnamoneus TH-2. A domain comprised of residues 140 to 154 is crucial for the catalytic activity of isoform EstA Streptomyces cinnamoneus
3.1.1.73 additional information construction of chimeric proteins by RIBS in vivo DNA shuffling of the feruloyl esterase genes of strains Streptomyces cinnamoneus NBRC 12852 and Streptomyces cinnamoneus TH-2. insertion of residues Pro and Gly between residues Asn-152 and Ala-153, i.e. mutant 18PG, leads to increased activity with substrates methyl ferulate and methyl sinapinate Streptomyces sp. TH-2
3.1.1.73 S191A mutation within catalytic triad, complete loss of activity Streptomyces cinnamoneus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 1.63
-
 methyl sinapinate wild-type, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 2 77 methyl sinapinate mutant 18PG, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 2.56
-
 methyl sinapinate pH not specified in the publication, 50°C Streptomyces cinnamoneus
3.1.1.73 2.77
-
 methyl ferulate mutant 18PG, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 3.72
-
 methyl ferulate pH not specified in the publication, 50°C Streptomyces cinnamoneus
3.1.1.73 4.13
-
 ethyl ferulate mutant 18PG, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 4.44
-
 ethyl ferulate wild-type, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 5.59
-
 ethyl ferulate pH not specified in the publication, 50°C Streptomyces cinnamoneus
3.1.1.73 9.66
-
 methyl ferulate wild-type, pH not specified in the publication, 50°C Streptomyces sp. TH-2

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Streptomyces cinnamoneus A0A0M4UW33
-
-
3.1.1.73 Streptomyces cinnamoneus NBRC 12852 A0A0M4UW33
-
-
3.1.1.73 Streptomyces sp. TH-2 A0A1E1FNX1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 ethyl ferulate + H2O
-
 Streptomyces cinnamoneus ferulate + ethanol
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Streptomyces sp. TH-2 ferulate + ethanol
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Streptomyces cinnamoneus NBRC 12852 ferulate + ethanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Streptomyces cinnamoneus ferulate + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Streptomyces sp. TH-2 ferulate + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Streptomyces cinnamoneus NBRC 12852 ferulate + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Streptomyces cinnamoneus sinapinate + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Streptomyces sp. TH-2 sinapinate + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Streptomyces cinnamoneus NBRC 12852 sinapinate + methanol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 EstA
-
 Streptomyces cinnamoneus
3.1.1.73 R18
-
 Streptomyces cinnamoneus
3.1.1.73 TH2-18
-
 Streptomyces sp. TH-2

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 45
-
-
 Streptomyces cinnamoneus
3.1.1.73 45
-
-
 Streptomyces sp. TH-2

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 60
-
 30 min, complete loss of activity Streptomyces cinnamoneus
3.1.1.73 60
-
 30 min, complete loss of activity Streptomyces sp. TH-2

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 0.0008
-
 methyl sinapinate wild-type, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 0.0017
-
 methyl sinapinate pH not specified in the publication, 50°C Streptomyces cinnamoneus
3.1.1.73 0.0027
-
 ethyl ferulate wild-type, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 0.0045
-
 methyl ferulate wild-type, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 0.0145
-
 methyl ferulate mutant 18PG, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 0.0283
-
 methyl ferulate pH not specified in the publication, 50°C Streptomyces cinnamoneus
3.1.1.73 0.0422
-
 ethyl ferulate pH not specified in the publication, 50°C Streptomyces cinnamoneus
3.1.1.73 0.073
-
 ethyl ferulate mutant 18PG, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 0.0732
-
 methyl sinapinate mutant 18PG, pH not specified in the publication, 50°C Streptomyces sp. TH-2

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6.5
-
 and 8.0 Streptomyces cinnamoneus
3.1.1.73 6.5
-
 and 8.0 Streptomyces sp. TH-2
3.1.1.73 8
-
 and 6.5 Streptomyces cinnamoneus
3.1.1.73 8
-
 and 6.5 Streptomyces sp. TH-2

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 0.00047
-
 methyl ferulate wild-type, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 0.0006
-
 methyl sinapinate pH not specified in the publication, 50°C Streptomyces cinnamoneus
3.1.1.73 0.0006
-
 methyl sinapinate wild-type, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 0.00062
-
 ethyl ferulate wild-type, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 0.00528
-
 methyl ferulate mutant 18PG, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 0.0076
-
 ethyl ferulate pH not specified in the publication, 50°C Streptomyces cinnamoneus
3.1.1.73 0.0077
-
 methyl ferulate pH not specified in the publication, 50°C Streptomyces cinnamoneus
3.1.1.73 0.0177
-
 ethyl ferulate mutant 18PG, pH not specified in the publication, 50°C Streptomyces sp. TH-2
3.1.1.73 0.0427
-
 methyl sinapinate mutant 18PG, pH not specified in the publication, 50°C Streptomyces sp. TH-2