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Literature summary extracted from

  • Saleh, M.A.; Han, W.J.; Lu, M.; Wang, B.; Li, H.; Kelly, R.M.; Li, F.L.
    Two Distinct alpha-L-arabinofuranosidases in Caldicellulosiruptor species drive degradation of arabinose-based polysaccharides (2017), Appl. Environ. Microbiol., 83, e00574-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expressed in Escherichia coli BL21(DE3) cells Caldicellulosiruptor sp. F32

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Co2+ 39% residual activity at 5 mM Caldicellulosiruptor sp. F32
3.2.1.55 Cu2+ 0.8% residual activity at 1 mM Caldicellulosiruptor sp. F32
3.2.1.55 EDTA the enzyme retains 24% activity in the presence of 5 mM EDTA Caldicellulosiruptor sp. F32
3.2.1.55 Fe2+ 16% residual activity at 1 mM Caldicellulosiruptor sp. F32
3.2.1.55 K+ 14% residual activity at 1 mM Caldicellulosiruptor sp. F32
3.2.1.55 Mg2+ 76% residual activity at 5 mM Caldicellulosiruptor sp. F32
3.2.1.55 Ni2+ 82% residual activity at 1 mM Caldicellulosiruptor sp. F32
3.2.1.55 SDS the enzyme is inactive in the presence of 5 mM SDS Caldicellulosiruptor sp. F32

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.6
-
 4-nitrophenyl alpha-L-arabinofuranoside at pH 6.0 and 80°C Caldicellulosiruptor sp. F32

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.55 cytosol
-
 Caldicellulosiruptor sp. F32 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.55 Ca2+ 106% activity at 5 mM Caldicellulosiruptor sp. F32
3.2.1.55 Mn2+ 117% activity at 5 mM Caldicellulosiruptor sp. F32

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 sugar beet arabinan + H2O Caldicellulosiruptor sp. F32 strong hydrolytic activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Caldicellulosiruptor sp. F32
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Caldicellulosiruptor sp. F32 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 sugar beet arabinan + H2O strong hydrolytic activity Caldicellulosiruptor sp. F32 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 AbF51
-
 Caldicellulosiruptor sp. F32
3.2.1.55 alpha-L-arabinofuranosidase
-
 Caldicellulosiruptor sp. F32

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 80
-
-
 Caldicellulosiruptor sp. F32

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 70 75 the half-lives of the enzyme at 70°C and 75°C are 100 h and 90 h, respectively Caldicellulosiruptor sp. F32

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.55 3900
-
 4-nitrophenyl alpha-L-arabinofuranoside at pH 6.0 and 80°C Caldicellulosiruptor sp. F32

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 6
-
-
 Caldicellulosiruptor sp. F32

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.55 6500
-
 4-nitrophenyl alpha-L-arabinofuranoside at pH 6.0 and 80°C Caldicellulosiruptor sp. F32