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Literature summary extracted from

  • Hobdey, S.E.; Knott, B.C.; Haddad Momeni, M.; Taylor, L.E.; Borisova, A.S.; Podkaminer, K.K.; VanderWall, T.A.; Himmel, M.E.; Decker, S.R.; Beckham, G.T.; Stahlberg, J.
    Biochemical and structural characterizations of two Dictyostelium cellobiohydrolases from the amoebozoa kingdom reveal a high level of conservation between distant phylogenetic trees of life (2016), Appl. Environ. Microbiol., 82, 3395-3409 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176 expressed in Trichoderma reesei Dictyostelium discoideum
3.2.1.176 expressed in Trichoderma reesei Dictyostelium purpureum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.176 sitting drop vapor diffusion method, using 0.1 M sodium malonate, pH 7.0 and 12% (w/v) PEG 3350 Dictyostelium purpureum
3.2.1.176 sitting drop vapor diffusion method, using 12.5% (w/v) polyethylene glycol 1000, 12.5% (w/v) PEG 3350, 12.5% (v/v) 2-methyl-2,4-pentanediol, 0.1 M sodium HEPES-MOPS pH 7.5, 0.03 M MgCl2, and 0.03 M CaCl2 Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.176 cellobiose competitive inhibition Dictyostelium discoideum
3.2.1.176 cellobiose competitive inhibition Dictyostelium purpureum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.176 3.4
-
 4-nitrophenyl beta-D-lactopyranoside at pH 5.0 and 40°C Dictyostelium discoideum
3.2.1.176 3.4
-
 4-nitrophenyl beta-D-lactopyranoside at pH 5.0 and 40°C Dictyostelium purpureum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.176 cellulose + H2O Dictyostelium discoideum
-
 cellobiose + ?
-
 ?
3.2.1.176 cellulose + H2O Dictyostelium purpureum
-
 cellobiose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Dictyostelium discoideum Q55FE6
-
-
3.2.1.176 Dictyostelium purpureum Q55FE6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.176 ammonium sulfate precipitation, phenyl Sepharose column chromatography, Source 15Q column chromatography, and Superdex 75 gel filtration Dictyostelium discoideum
3.2.1.176 ammonium sulfate precipitation, phenyl Sepharose column chromatography, Source 15Q column chromatography, and Superdex 75 gel filtration Dictyostelium purpureum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 4-nitrophenyl beta-D-lactopyranoside + H2O
-
 Dictyostelium discoideum 4-nitrophenol + beta-D-lactose
-
 ?
3.2.1.176 4-nitrophenyl beta-D-lactopyranoside + H2O
-
 Dictyostelium purpureum 4-nitrophenol + beta-D-lactose
-
 ?
3.2.1.176 avicel + H2O
-
 Dictyostelium discoideum ?
-
 ?
3.2.1.176 avicel + H2O
-
 Dictyostelium purpureum ?
-
 ?
3.2.1.176 cellulose + H2O
-
 Dictyostelium discoideum cellobiose + ?
-
 ?
3.2.1.176 cellulose + H2O
-
 Dictyostelium purpureum cellobiose + ?
-
 ?
3.2.1.176 phosphoric acid-swollen cellulose + H2O
-
 Dictyostelium discoideum ?
-
 ?
3.2.1.176 phosphoric acid-swollen cellulose + H2O
-
 Dictyostelium purpureum ?
-
 ?
3.2.1.176 pretreated corn stover + H2O
-
 Dictyostelium discoideum ?
-
 ?
3.2.1.176 pretreated corn stover + H2O
-
 Dictyostelium purpureum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 Cel7A
-
 Dictyostelium discoideum
3.2.1.176 Cel7A
-
 Dictyostelium purpureum
3.2.1.176 cellobiohydrolase
-
 Dictyostelium discoideum
3.2.1.176 cellobiohydrolase
-
 Dictyostelium purpureum
3.2.1.176 GH7 CBH
-
 Dictyostelium discoideum
3.2.1.176 GH7 CBH
-
 Dictyostelium purpureum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.176 45
-
-
 Dictyostelium discoideum
3.2.1.176 55
-
-
 Dictyostelium purpureum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.176 53
-
 melting temperature Dictyostelium discoideum
3.2.1.176 62
-
 melting temperature Dictyostelium purpureum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.176 0.27
-
 4-nitrophenyl beta-D-lactopyranoside at pH 5.0 and 40°C Dictyostelium discoideum
3.2.1.176 0.6
-
 4-nitrophenyl beta-D-lactopyranoside at pH 5.0 and 40°C Dictyostelium purpureum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.176 5
-
-
 Dictyostelium discoideum
3.2.1.176 5
-
-
 Dictyostelium purpureum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.176 0.13
-
 cellobiose at pH 5.0 and 40°C Dictyostelium purpureum
3.2.1.176 0.205
-
 cellobiose at pH 5.0 and 40°C Dictyostelium discoideum