EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.14.11 | synthesis | in an organic solvent-water biphasic reaction system, recombinant enzyme enantioselectively converts linear terminal alkenes to their corresponding (S)-epoxyalkanes using glucose and molecular oxygen. When 1-heptene and 6-chloro-1-hexene are used as substrates (400 mM) under optimized conditions, 88.3mM (S)-1,2-epoxyheptane and 246.5mM (S)-1,2-epoxy-6-chlorohexane, respectively, accumulate in the organic phase with good enantiomeric excess (84.2% and 95.5%, respectively) | Rhodococcus sp. ST-10 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.11 | expression in Kocuria rhizophila DC2201 | Rhodococcus sp. ST-10 |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.14.14.11 | additional information | for epoxyalkane production in a biophasic system, di-n-octyl phthalate and bis(2-ethylhexyl)phthalate are the best solvents. Phthalate esters, except methyl phthalate, produce higher levels of (S)-1,2-epoxyheptane than medium- or long-chain (C10 to C18) alkanes | Rhodococcus sp. ST-10 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.11 | Rhodococcus sp. ST-10 | G3XEX2 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.11 | 2-methylhex-1-ene + FADH2 + O2 | - |
Rhodococcus sp. ST-10 | (S)-1,2-epoxy-2-methylhexane + FAD + H2O | - |
? | |
1.14.14.11 | 6-bromohex-1-ene + FADH2 + O2 | - |
Rhodococcus sp. ST-10 | (S)-1,2-epoxy-6-bromohexane + FAD + H2O | - |
? | |
1.14.14.11 | 6-chlorohex-1-ene + FADH2 + O2 | - |
Rhodococcus sp. ST-10 | (S)-1,2-epoxy-6-chlorohexane + FAD + H2O | - |
? | |
1.14.14.11 | hept-1-ene + FADH2 + O2 | - |
Rhodococcus sp. ST-10 | (S)-1,2-epoxy-2-methylhexane + FAD + H2O | - |
? | |
1.14.14.11 | additional information | upon reaction in an organic solvent-water biphasic reaction system, the highest production level (246.5 mM) is achieved for 6-chloro-1-hexene, followed by styrene, 6-bromo-1-hexene, 2-methyl-1-hexene, 1-heptene, and 5-hexenenitrile in decreasing order | Rhodococcus sp. ST-10 | ? | - |
? | |
1.14.14.11 | styrene + FADH2 + O2 | - |
Rhodococcus sp. ST-10 | (S)-styrene oxide + FAD + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.11 | StyA | - |
Rhodococcus sp. ST-10 |