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Literature summary extracted from
- Rubber oxygenase and latex clearing protein cleave rubber to different products and use different cleavage mechanisms (2014), Appl. Environ. Microbiol., 80, 5012-5020 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.11.87 | expression of Lcp of Streptomyces sp. K30 in a DELTAroxA background of Xanthomonas sp. strain 35Y | Streptomyces sp. K30 |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.87 | alpha-tocopherol | 1 mM, 40% inhibition | Streptomyces sp. K30 |  |
| 1.13.11.87 | ascorbate | 1 mM, 20% inhibition | Streptomyces sp. K30 | |
| 1.13.11.87 | carotene | 1 mM, 40% inhibition | Streptomyces sp. K30 | |
| 1.13.11.87 | diethyldithiocarbamate | 1 mM, 50% inhibition | Streptomyces sp. K30 | |
| 1.13.11.87 | dithiothreitol | 10 mM, 90% inhibition | Streptomyces sp. K30 | |
| 1.13.11.87 | EDTA | 1 mM, 30% inhibition | Streptomyces sp. K30 | |
| 1.13.11.87 | ethanol | 5%, 10% inhibition | Streptomyces sp. K30 | |
| 1.13.11.87 | imidazole | inhibits by 35% and 85% at 1 mM and 10 mM | Streptomyces sp. K30 | |
| 1.13.11.87 | Phenanthroline | 1 mM, 10% inhibition | Streptomyces sp. K30 | |
| 1.13.11.87 | SDS | 0.1%, complete inhibition | Streptomyces sp. K30 | |
| 1.13.11.87 | squalene | 1 mM, 10% inhibition | Streptomyces sp. K30 | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.13.11.85 | extracellular | - |
Xanthomonas sp. 35Y | - |
- |
| 1.13.11.87 | extracellular | - |
Streptomyces sp. K30 | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.87 | additional information | no indication for the presence of iron, other metals, or cofactors in Lcp | Streptomyces sp. K30 |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.87 | 40000 | - |
SDS-PAGE | Streptomyces sp. K30 |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.85 | cis-1,4-polyisoprene + O2 | Xanthomonas sp. 35Y | - |
(4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.85 | Xanthomonas sp. 35Y | Q7X0P3 | - |
- |
| 1.13.11.87 | Streptomyces sp. K30 | Q3L8N0 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.85 | - |
Xanthomonas sp. 35Y |
| 1.13.11.87 | - |
Streptomyces sp. K30 |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.13.11.87 | almost stable during storage on ice | Streptomyces sp. K30 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.85 | cis-1,4-polyisoprene + O2 | - |
Xanthomonas sp. 35Y | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
| 1.13.11.85 | cis-1,4-polyisoprene + O2 | exo-type cleavage mechanism. RoxA is able to cleave isolated latex-clearing protein-derived oligo-isoprenoid molecules to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal groups | Xanthomonas sp. 35Y | (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal | - |
? | |
| 1.13.11.87 | cis-1,4-polyisoprene + O2 | Lcp degrades polyisoprene via endo-type cleavage to tetra-C20 and higher oligo-isoprenoids with aldehyde and keto end groups | Streptomyces sp. K30 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.87 | ? | x * 40000, SDS-PAGE | Streptomyces sp. K30 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.85 | roxA | - |
Xanthomonas sp. 35Y |
| 1.13.11.87 | latex-clearing protein | - |
Streptomyces sp. K30 |
| 1.13.11.87 | Lcp | - |
Streptomyces sp. K30 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.85 | 25 | - |
assay at | Xanthomonas sp. 35Y |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.85 | 23 | 30 | at 30°C, the specific activity of RoxA is almost double as high as at 23°C | Xanthomonas sp. 35Y |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.85 | 37 | - |
no significant reduction in activity after 22 h | Xanthomonas sp. 35Y |
| 1.13.11.87 | 23 | - |
Lcp activity decreases by incubation at 23°C by 70% within the 22 h of incubation time | Streptomyces sp. K30 |
| 1.13.11.87 | 37 | - |
Lcp activity decreases by incubation at 37°C by 80% within the 22 h of incubation time | Streptomyces sp. K30 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.85 | 6 | 8 | in potassium phosphate buffer or in HEPES buffer. Other buffers (Tris, carbonate-ammonium acetate, and glycylglycine) result in poor activities | Xanthomonas sp. 35Y |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.85 | 6 | 8.5 | pH values below pH 6 or above 8.5 result in only poor activities | Xanthomonas sp. 35Y |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.85 | heme | - |
Xanthomonas sp. 35Y | |
| 1.13.11.87 | additional information | no indication for the presence of iron, other metals, or cofactors in Lcp | Streptomyces sp. K30 |
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Release 2023.1 (January 2023)
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