EC Number | Cloned (Comment) | Organism |
---|---|---|
1.15.1.1 | AhSOD DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Amaranthus hypochondriacus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | H2O2 | - |
Amaranthus hypochondriacus | |
1.15.1.1 | KCN | - |
Amaranthus hypochondriacus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | Cu2+ | a Cu/ZnSOD, highly conserved amino acid residues are involved in Cu/Zn binding | Amaranthus hypochondriacus | |
1.15.1.1 | additional information | enzyme affinities for copper, zinc, and nickel, overview | Amaranthus hypochondriacus | |
1.15.1.1 | Zn2+ | a Cu/ZnSOD, highly conserved amino acid residues are involved in Cu/Zn binding | Amaranthus hypochondriacus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 28500 | - |
recombinant His-tagged enzyme, gel filtration | Amaranthus hypochondriacus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.15.1.1 | 2 superoxide + 2 H+ | Amaranthus hypochondriacus | - |
O2 + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.15.1.1 | Amaranthus hypochondriacus | F6JRN6 | cv. Nutrisol | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.15.1.1 | recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and dialysis, anion exchange chromatography and dialysis, and ultrafiltration | Amaranthus hypochondriacus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.15.1.1 | root | - |
Amaranthus hypochondriacus | - |
1.15.1.1 | seedling | - |
Amaranthus hypochondriacus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.15.1.1 | 2 superoxide + 2 H+ | - |
Amaranthus hypochondriacus | O2 + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.15.1.1 | homodimer | 2 * 15600, about, sequence calculation | Amaranthus hypochondriacus |
1.15.1.1 | More | enzyme structural analysis by circular dichroism analysis, recombinnat AhSOD is an intrinsically disorder enzyme, sequence comparisons and three-dimensional model | Amaranthus hypochondriacus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.15.1.1 | AhSOD | - |
Amaranthus hypochondriacus |
1.15.1.1 | Cu/Zn superoxide dismutase | - |
Amaranthus hypochondriacus |
1.15.1.1 | Cu/ZnSOD | - |
Amaranthus hypochondriacus |
1.15.1.1 | SOD | - |
Amaranthus hypochondriacus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 37 | - |
assay at | Amaranthus hypochondriacus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 8 | - |
assay at | Amaranthus hypochondriacus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.15.1.1 | Amaranthus hypochondriacus | sequence calculation | - |
5.4 |