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Literature summary extracted from

  • Noori, A.R.; Hosseinkhani, S.; Ghiasi, P.; Akbari, J.; Heydari, A.
    Magnetic nanoparticles supported ionic liquids improve firefly luciferase properties (2014), Appl. Biochem. Biotechnol., 172, 3116-3127 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.7 additional information enzyme activity and structural stability increases in presence of magnetic magnetite (lambda-Fe2O3) nanoparticles supported ionic liquids. The effect of ingredients which are used is not considerable on Km value of luciferase for ATP and also Km value for luciferin, synthesis and evaluation, overview. The use of ionic liquids supported on magnetic nanoparticles improves kinetic and structural properties of firefly luciferase using bioluminescence assay, fluorescence spectroscopy, and circular dichroism Photinus pyralis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.7 additional information irreversible aggregation because of the exposure of its hydrophobic sites followed by structural changes leads to its further inactivation Photinus pyralis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.7 D-firefly luciferin + O2 + ATP Photinus pyralis
-
 firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Photinus pyralis P08659
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 D-firefly luciferin + O2 + ATP
-
 Photinus pyralis firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.7 firefly luciferase
-
 Photinus pyralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.7 25
-
 free wild-type enzyme Photinus pyralis
1.13.12.7 30
-
 enzyme in presence of magnetic magnetite nanoparticles supported ionic liquids Photinus pyralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.12.7 additional information
-
 thermal stability and activity at 35°C of the enzyme in presence of magnetic magnetite nanoparticles supported ionic liquids is increased compared to the free enzyme, overview Photinus pyralis
1.13.12.7 35 45 free enzyme shows 65% remaning activity after 10 min at 35°C, inactivation after 4 min at 35°C, inactivation at 45°C Photinus pyralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.7 7.8
-
-
 Photinus pyralis

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.7 ATP
-
 Photinus pyralis

General Information

EC Number General Information Comment Organism
1.13.12.7 physiological function firefly luciferase from North American Photinus pyralis is a monooxygenase that performs ATP-dependent conversion of luciferin into a luciferyl-adenylate, which is oxidized in a multistep reaction to electronically excited oxyluciferin Photinus pyralis