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Literature summary extracted from

  • Kim, D.; Lee, M.; Cho, H.; Lee, J.; Lee, M.; Chung, C.; Shin, D.; Rhee, Y.; Son, K.; Park, H.
    Genetic and functional characterization of an extracellular modular GH6 endo-beta-1,4-glucanase from an earthworm symbiont, Cellulosimicrobium funkei HY-13 (2016), Antonie van Leeuwenhoek, 109, 1-12 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.6 expression in Escherichia coli Cellulosimicrobium funkei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.6 Hg2+ 1 mM, slight inhibition Cellulosimicrobium funkei
3.2.1.6 N-bromosuccinimide 10 min, 95% loss of activity Cellulosimicrobium funkei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.6 Ca2+ 1 mM, 1.2fold increase in activity Cellulosimicrobium funkei
3.2.1.6 Co2+ 1 mM, 1.4fold increase in activity Cellulosimicrobium funkei
3.2.1.6 Mn2+ 1 mM, 1.2fold increase in activity Cellulosimicrobium funkei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Cellulosimicrobium funkei A0A0P0IZM9
-
-
3.2.1.6 Cellulosimicrobium funkei HY-13 A0A0P0IZM9
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.6 2.8
-
 substrate carboxymethylcellulose, pH 5.0, 50°C Cellulosimicrobium funkei
3.2.1.6 6.2
-
 substrate barley beta-1,3-1,4-D-glucan, pH 5.0, 50°C Cellulosimicrobium funkei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 avicel + H2O
-
 Cellulosimicrobium funkei ?
-
 ?
3.2.1.6 avicel + H2O
-
 Cellulosimicrobium funkei HY-13 ?
-
 ?
3.2.1.6 barley beta-1,3-1,4-D-glucan + H2O
-
 Cellulosimicrobium funkei cellotriose
-
 ?
3.2.1.6 carboxymethylcellulose + H2O 45% of the activity with barley beta-1,3-1,4-D-glucan Cellulosimicrobium funkei ?
-
 ?
3.2.1.6 carboxymethylcellulose + H2O 45% of the activity with barley beta-1,3-1,4-D-glucan Cellulosimicrobium funkei HY-13 ?
-
 ?
3.2.1.6 cellopentaose + H2O
-
 Cellulosimicrobium funkei cellobiose + cellotriose
-
 ?
3.2.1.6 cellopentaose + H2O
-
 Cellulosimicrobium funkei HY-13 cellobiose + cellotriose
-
 ?
3.2.1.6 konjac glucomannan + H2O 40% of the activity with barley beta-1,3-1,4-D-glucan Cellulosimicrobium funkei ?
-
 ?
3.2.1.6 lichenan + H2O 48% of the activity with barley beta-1,3-1,4-D-glucan Cellulosimicrobium funkei ?
-
 ?
3.2.1.6 lichenan + H2O 48% of the activity with barley beta-1,3-1,4-D-glucan Cellulosimicrobium funkei HY-13 ?
-
 ?
3.2.1.6 additional information activity in decreasing order is barley beta-1,3-1,4-D-glucan, carboxymethylcellulose, lichenan, avicel, konjac glucomannan. No substrates: cellobiose, cellotriose, p-nitrophenyl derivatives of mono- and disaccharides, or beta-1,3-D-glucan, beta-1,4-D-galactomannan, and beta-1,4-D-xylan Cellulosimicrobium funkei ?
-
 ?
3.2.1.6 additional information activity in decreasing order is barley beta-1,3-1,4-D-glucan, carboxymethylcellulose, lichenan, avicel, konjac glucomannan. No substrates: cellobiose, cellotriose, p-nitrophenyl derivatives of mono- and disaccharides, or beta-1,3-D-glucan, beta-1,4-D-galactomannan, and beta-1,4-D-xylan Cellulosimicrobium funkei HY-13 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.6 ? x * 56000, calculated and SDS-PAGE Cellulosimicrobium funkei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 CelL
-
 Cellulosimicrobium funkei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.6 50
-
-
 Cellulosimicrobium funkei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.6 50
-
 half-life 12 min Cellulosimicrobium funkei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.6 5
-
-
 Cellulosimicrobium funkei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.6 4 10 1 h, 90% residual activity Cellulosimicrobium funkei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.6 Cellulosimicrobium funkei calculated
-
 4.5