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Literature summary extracted from

  • Fricke, J.; Blei, F.; Hoffmeister, D.
    Enzymatic synthesis of psilocybin (2017), Angew. Chem. Int. Ed. Engl., 56, 12352-12355 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.59 expressed in Aspergillus niger strain tJF02.05 Psilocybe cubensis
2.1.1.345 expressed in Escherichia coli KRX cells Psilocybe cubensis
2.7.1.222 expressed in Escherichia coli KRX cells Psilocybe cubensis
4.1.1.105 expressed in Escherichia coli KRX cells Psilocybe cubensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.59 N,N-dimethyltryptamine + reduced acceptor + O2 Psilocybe cubensis
-
 psilocin + acceptor + H2O
-
 ?
1.14.99.59 tryptamine + reduced acceptor + O2 Psilocybe cubensis
-
 4-hydroxytryptamine + acceptor + H2O
-
 ?
2.1.1.345 2 S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate Psilocybe cubensis
-
 2 S-adenosyl-L-homocysteine + psilocybin
-
 ?
2.1.1.345 S-adenosyl-L-methionine + 4-hydroxy-N-methyltryptamine 4-phosphate Psilocybe cubensis
-
 S-adenosyl-L-homocysteine + psilocybin
-
 ?
2.1.1.345 S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate Psilocybe cubensis
-
 S-adenosyl-L-homocysteine + 4-hydroxy-N-methyltryptamine 4-phosphate
-
 ?
2.7.1.222 ATP + 4-hydroxytryptamine Psilocybe cubensis
-
 ADP + norbaeocystin
-
 ?
2.7.1.222 ATP + psilocin Psilocybe cubensis
-
 ADP + psilocybin
-
 ?
4.1.1.105 L-tryptophan Psilocybe cubensis
-
 tryptamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.59 Psilocybe cubensis P0DPA7
-
-
2.1.1.345 Psilocybe cubensis P0DPA9
-
-
2.7.1.222 Psilocybe cubensis P0DPA8
-
-
4.1.1.105 Psilocybe cubensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.59 metal affinity column chromatography Psilocybe cubensis
2.1.1.345 metal affinity column chromatography Psilocybe cubensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.59 N,N-dimethyltryptamine + reduced acceptor + O2
-
 Psilocybe cubensis psilocin + acceptor + H2O
-
 ?
1.14.99.59 tryptamine + reduced acceptor + O2
-
 Psilocybe cubensis 4-hydroxytryptamine + acceptor + H2O
-
 ?
2.1.1.345 2 S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate
-
 Psilocybe cubensis 2 S-adenosyl-L-homocysteine + psilocybin
-
 ?
2.1.1.345 S-adenosyl-L-methionine + 4-hydroxy-N-methyltryptamine 4-phosphate
-
 Psilocybe cubensis S-adenosyl-L-homocysteine + psilocybin
-
 ?
2.1.1.345 S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate
-
 Psilocybe cubensis S-adenosyl-L-homocysteine + 4-hydroxy-N-methyltryptamine 4-phosphate
-
 ?
2.1.1.345 S-adenosyl-L-methionine + baeocystin
-
 Psilocybe cubensis S-adenosyl-L-homocysteine + psilocybin
-
 ?
2.1.1.345 S-adenosyl-L-methionine + norbaeocystin
-
 Psilocybe cubensis S-adenosyl-L-homocysteine + baeocystin
-
 ?
2.7.1.222 ATP + 4-hydroxytryptamine
-
 Psilocybe cubensis ADP + norbaeocystin
-
 ?
2.7.1.222 ATP + psilocin
-
 Psilocybe cubensis ADP + psilocybin
-
 ?
4.1.1.105 L-tryptophan
-
 Psilocybe cubensis tryptamine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.345 ? x * 34500, calculated from amino acid sequence Psilocybe cubensis
2.7.1.222 ? x * 40400, calculated from amino acid sequence Psilocybe cubensis
4.1.1.105 ? x * 49600, calculated from amino acid sequence Psilocybe cubensis

Synonyms

EC Number Synonyms Comment Organism
1.14.99.59 PsiH
-
 Psilocybe cubensis
2.1.1.345 PsiM
-
 Psilocybe cubensis
2.7.1.222 PsiK
-
 Psilocybe cubensis
4.1.1.105 psiD
-
 Psilocybe cubensis

General Information

EC Number General Information Comment Organism
1.14.99.59 metabolism the enzyme is involved in psilocybin synthesis Psilocybe cubensis
2.1.1.345 metabolism the enzyme is involved in psilocybin synthesis Psilocybe cubensis