Literature summary extracted from

Schnepel, C.; Minges, H.; Frese, M.; Sewald, N.
A high-throughput fluorescence assay to determine the activity of tryptophan halogenases (2016), Angew. Chem. Int. Ed. Engl., 55, 14159-14163 .

Application

EC Number	Application	Comment	Organism
1.14.19.59	analysis	development of a quantitative halogenase assay based on a Suzuki-Miyaura cross-coupling towards the formation of a fluorescent aryltryptophan and optimization for application in crude Escherichia coli lysate	Streptomyces albogriseolus

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.14.19.59	K374R	thermostable tryptophan 6-halogenase mutant, increase in melting temperature by 10 degrees	Streptomyces albogriseolus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.19.59	Streptomyces albogriseolus	A1E280	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.19.59	L-tryptophan + FADH2 + bromide + O2 + H+	-	Streptomyces albogriseolus	6-bromo-L-tryptophan + FAD + 2 H2O	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.19.59	Thal	-	Streptomyces albogriseolus

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
1.14.19.59	45	-	wild-type, inactive above	Streptomyces albogriseolus
1.14.19.59	49	-	mutant K374R, 99% of maximum activity	Streptomyces albogriseolus
1.14.19.59	56	-	mutant K374R, 71% of maximum activity	Streptomyces albogriseolus

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
1.14.19.59	47	-	melting temperature, wild-type	Streptomyces albogriseolus
1.14.19.59	57	-	melting temperature, mutant K374R	Streptomyces albogriseolus

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Release 2023.1 (January 2023)