BRENDA - Enzyme Database

Tris(3-hydroxypropyl)phosphine is superior to dithiothreitol for in vitro assessment of vitamin K 2,3-epoxide reductase activity

Krettler, C.; Bevans, C.G.; Reinhart, C.; Watzka, M.; Oldenburg, J.; Anal. Biochem. 474, 89-94 (2015) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.17.4.4
expression in Pichia pastoris
Homo sapiens
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.17.4.4
Homo sapiens
Q9BQB6
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.17.4.4
2,3-epoxy-2-methyl-3-phytyl-2,3-dihydro-1,4-naphthoquinone + tris(3-hydroxypropyl)phosphine
by replacing dithiothreitol with tris(3-hydroxypropyl)phosphine and replacing imidazole with phosphate as pH buffer, all nonenzymatic side reactions are effectively eliminated and accurate measurement of enzymatic activity in vitro is possible
744010
Homo sapiens
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.17.4.4
vitamin K 2,3-epoxide reductase
-
Homo sapiens
1.17.4.4
VKOR
-
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.17.4.4
25
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.4.4
7.4
-
assay at
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.4.4
expression in Pichia pastoris
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.17.4.4
2,3-epoxy-2-methyl-3-phytyl-2,3-dihydro-1,4-naphthoquinone + tris(3-hydroxypropyl)phosphine
by replacing dithiothreitol with tris(3-hydroxypropyl)phosphine and replacing imidazole with phosphate as pH buffer, all nonenzymatic side reactions are effectively eliminated and accurate measurement of enzymatic activity in vitro is possible
744010
Homo sapiens
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.17.4.4
25
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.4.4
7.4
-
assay at
Homo sapiens