Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mangan, D.; Cornaggia, C.; McKie, V.; Kargelis, T.; McCleary, B.V.
    A novel automatable enzyme-coupled colorimetric assay for endo-1,4-beta-glucanase (cellulase) (2016), Anal. Bioanal. Chem., 408, 4159-4168 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.4 analysis rapid, selective, quantitative assay based on substrate 44-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside and reaction of product 4,6-O-(3-oxobutylidene)-beta-D-cellotriose with beta-glucosidase Trichoderma longibrachiatum
3.2.1.4 analysis rapid, selective, quantitative assay based on substrate 44-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside and reaction of product 4,6-O-(3-oxobutylidene)-beta-D-cellotriose with beta-glucosidase Bacillus amyloliquefaciens
3.2.1.4 analysis rapid, selective, quantitative assay based on substrate 44-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside and reaction of product 4,6-O-(3-oxobutylidene)-beta-D-cellotriose with beta-glucosidase Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Aspergillus niger O74705
-
-
3.2.1.4 Bacillus amyloliquefaciens Q8RPQ6
-
-
3.2.1.4 Trichoderma longibrachiatum Q12714
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 commercial preparation
-
 Trichoderma longibrachiatum
-
3.2.1.4 commercial preparation
-
 Bacillus amyloliquefaciens
-
3.2.1.4 commercial preparation
-
 Aspergillus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside + H2O
-
 Trichoderma longibrachiatum 4,6-O-(3-oxobutylidene)-beta-D-cellotriose + 4-nitrophenyl-beta-D-cellobioside
-
 ?
3.2.1.4 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside + H2O
-
 Bacillus amyloliquefaciens 4,6-O-(3-oxobutylidene)-beta-D-cellotriose + 4-nitrophenyl-beta-D-cellobioside
-
 ?
3.2.1.4 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside + H2O
-
 Aspergillus niger 4,6-O-(3-oxobutylidene)-beta-D-cellotriose + 4-nitrophenyl-beta-D-cellobioside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Egl1
-
 Trichoderma longibrachiatum
3.2.1.4 EglA
-
 Aspergillus niger
3.2.1.4 EngA
-
 Bacillus amyloliquefaciens