EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.B26 | expressed in Escherichia coli strain ER2566 | Saccharolobus solfataricus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.B26 | F222A | the mutant shows 105% activity towards ginsenoside Rd compared to the wild type enzyme | Saccharolobus solfataricus |
3.2.1.B26 | F359A | the mutant shows 84% activity towards ginsenoside Rd compared to the wild type enzyme | Saccharolobus solfataricus |
3.2.1.B26 | L213A | the mutant shows 110% activity with ginsenoside Rd compared to the wild type enzyme | Saccharolobus solfataricus |
3.2.1.B26 | S220A | the mutant shows 92% activity towards ginsenoside Rd compared to the wild type enzyme | Saccharolobus solfataricus |
3.2.1.B26 | V209A | the mutant shows 170% activity towards ginsenoside Rd compared to the wild type enzyme | Saccharolobus solfataricus |
3.2.1.B26 | V209D | inactive | Saccharolobus solfataricus |
3.2.1.B26 | V209F | inactive | Saccharolobus solfataricus |
3.2.1.B26 | V209G | the mutant shows 60% activity towards ginsenoside Rd compared to the wild type enzyme | Saccharolobus solfataricus |
3.2.1.B26 | V209K | inactive | Saccharolobus solfataricus |
3.2.1.B26 | V209L | the mutant shows 10% activity towards ginsenoside Rd compared to the wild type enzyme | Saccharolobus solfataricus |
3.2.1.B26 | V209T | the mutant shows 80% activity towards ginsenoside Rd compared to the wild type enzyme | Saccharolobus solfataricus |
3.2.1.B26 | V209W | inactive | Saccharolobus solfataricus |
3.2.1.B26 | W361A | the mutant shows 241% activity towards ginsenoside Rd compared to the wild type enzyme | Saccharolobus solfataricus |
3.2.1.B26 | W361F | the mutant exhibits 4.2fold higher activity, 3.7fold higher catalytic efficiency, and 3.1fold lower binding energy for ginsenoside Rd than the wild type enzyme. The mutant completely converts ginsenoside Rb1 to compound K and shows also 7.4fold higher activity for hesperidin than the wild type enzyme | Saccharolobus solfataricus |
3.2.1.B26 | W361G | the mutant shows about 290% activity towards ginsenoside Rd compared to the wild type enzyme | Saccharolobus solfataricus |
3.2.1.B26 | W361L | the mutant shows about 40% activity towards ginsenoside Rd compared to the wild type enzyme | Saccharolobus solfataricus |
3.2.1.B26 | W361T | the mutant shows about 350% activity towards ginsenoside Rd compared to the wild type enzyme | Saccharolobus solfataricus |
3.2.1.B26 | W361Y | the mutant shows about 230% activity towards ginsenoside Rd compared to the wild type enzyme | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B26 | 0.52 | - |
ginsenoside Rd | wild type enzyme, at pH 5.5 and 95°C | Saccharolobus solfataricus | |
3.2.1.B26 | 0.57 | - |
ginsenoside Rb1 | wild type enzyme, at pH 5.5 and 95°C | Saccharolobus solfataricus | |
3.2.1.B26 | 1.59 | - |
ginsenoside Rd | mutant enzyme W361F, at pH 5.5 and 95°C | Saccharolobus solfataricus | |
3.2.1.B26 | 1.93 | - |
ginsenoside Rb1 | mutant enzyme W361F, at pH 5.5 and 95°C | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.B26 | Saccharolobus solfataricus | P22498 | - |
- |
3.2.1.B26 | Saccharolobus solfataricus DSM 1617 | P22498 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.B26 | His-Trap column chromatography, and Superdex 200 gel filtration | Saccharolobus solfataricus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | 0.0019 | - |
wild type enzyme, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 0.0072 | - |
mutant enzyme W361F, with ginsenoside C-Mc as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 0.012 | - |
mutant enzyme W361F, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 0.0154 | - |
wild type enzyme, with ginsenoside C-Mc as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 0.0201 | - |
wild type enzyme, with ginsenoside Rc as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 0.0233 | - |
mutant enzyme W361F, with ginsenoside Rc as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 0.0355 | - |
mutant enzyme W361F, with naringin as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 0.0391 | - |
wild type enzyme, with hesperidin as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 0.233 | - |
wild type enzyme, with naringin as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 0.288 | - |
mutant enzyme W361F, with hesperidin as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 0.338 | - |
wild type enzyme, with ginsenoside Rd as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 1.347 | - |
mutant enzyme W361F, with ginsenoside C-Y as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 1.415 | - |
mutant enzyme W361F, with ginsenoside Rd as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 1.643 | - |
wild type enzyme, with ginsenoside Rb2 as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 2.031 | - |
mutant enzyme W361F, with ginsenoside C-O as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 3.213 | - |
mutant enzyme W361F, with ginsenoside Rb1 as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 4.388 | - |
wild type enzyme, with ginsenoside C-Y as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 5.082 | - |
wild type enzyme, with ginsenoside Rb1 as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 6.354 | - |
wild type enzyme, with ginsenoside C-O as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 9.077 | - |
mutant enzyme W361F, with ginsenoside Rb2 as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 13.13 | - |
wild type enzyme, with ginsenoside F2 as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
3.2.1.B26 | 13.227 | - |
mutant enzyme W361F, with ginsenoside F2 as substrate, at pH 5.5 and 95°C | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.B26 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + D-glucose | - |
? | |
3.2.1.B26 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Saccharolobus solfataricus DSM 1617 | 4-nitrophenol + D-glucose | - |
? | |
3.2.1.B26 | ginsenoside C-Mc + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.2.1.B26 | ginsenoside C-Mc + H2O | - |
Saccharolobus solfataricus DSM 1617 | ? | - |
? | |
3.2.1.B26 | ginsenoside C-Mc1 + H2O | - |
Saccharolobus solfataricus | ginsenoside C-K + D-glucose + L-arabinofuranose | - |
? | |
3.2.1.B26 | ginsenoside C-Mc1 + H2O | - |
Saccharolobus solfataricus DSM 1617 | ginsenoside C-K + D-glucose + L-arabinofuranose | - |
? | |
3.2.1.B26 | ginsenoside C-O + H2O | - |
Saccharolobus solfataricus | ginsenoside C-K + D-glucose + L-arabinopyranose | - |
? | |
3.2.1.B26 | ginsenoside C-Y + H2O | - |
Saccharolobus solfataricus | ginsenoside C-K + L-arabinopyranose | - |
? | |
3.2.1.B26 | ginsenoside F2 + H2O | highest activity | Saccharolobus solfataricus | ginsenoside C-K + D-glucose | - |
? | |
3.2.1.B26 | ginsenoside Rb1 + H2O | - |
Saccharolobus solfataricus | ginsenoside C-K + D-glucose | - |
? | |
3.2.1.B26 | ginsenoside Rb2 + H2O | - |
Saccharolobus solfataricus | ginsenoside C-K + D-glucose + L-arabinopyranose | - |
? | |
3.2.1.B26 | ginsenoside Rc + H2O | - |
Saccharolobus solfataricus | ginsenoside C-K + D-glucose + L-arabinofuranose | - |
? | |
3.2.1.B26 | ginsenoside Rd + H2O | - |
Saccharolobus solfataricus | ginsenoside C-K + ? | - |
? | |
3.2.1.B26 | ginsenoside Rd + H2O | - |
Saccharolobus solfataricus | ginsenoside C-K + D-glucose | - |
? | |
3.2.1.B26 | hesperidin + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.2.1.B26 | hesperidin + H2O | - |
Saccharolobus solfataricus DSM 1617 | ? | - |
? | |
3.2.1.B26 | naringin + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.2.1.B26 | naringin + H2O | - |
Saccharolobus solfataricus DSM 1617 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.B26 | beta-glycosidase | - |
Saccharolobus solfataricus |
3.2.1.B26 | Bgl | - |
Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B26 | 92 | - |
ginsenoside Rd | wild type enzyme, at pH 5.5 and 95°C | Saccharolobus solfataricus | |
3.2.1.B26 | 1049 | - |
ginsenoside Rd | mutant enzyme W361F, at pH 5.5 and 95°C | Saccharolobus solfataricus | |
3.2.1.B26 | 1343 | - |
ginsenoside Rb1 | mutant enzyme W361F, at pH 5.5 and 95°C | Saccharolobus solfataricus | |
3.2.1.B26 | 2257 | - |
ginsenoside Rb1 | wild type enzyme, at pH 5.5 and 95°C | Saccharolobus solfataricus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B26 | 178 | - |
ginsenoside Rd | wild type enzyme, at pH 5.5 and 95°C | Saccharolobus solfataricus | |
3.2.1.B26 | 660 | - |
ginsenoside Rd | mutant enzyme W361F, at pH 5.5 and 95°C | Saccharolobus solfataricus | |
3.2.1.B26 | 697 | - |
ginsenoside Rb1 | mutant enzyme W361F, at pH 5.5 and 95°C | Saccharolobus solfataricus | |
3.2.1.B26 | 3967 | - |
ginsenoside Rb1 | wild type enzyme, at pH 5.5 and 95°C | Saccharolobus solfataricus |