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Literature summary extracted from

  • Okai, N.; Masuda, T.; Takeshima, Y.; Tanaka, K.; Yoshida, K.I.; Miyamoto, M.; Ogino, C.; Kondo, A.
    Biotransformation of ferulic acid to protocatechuic acid by Corynebacterium glutamicum ATCC 21420 engineered to express vanillate O-demethylase (2017), AMB Express, 7, 130 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.82 gene vanAB, recombinant overexpression in Corynebacterium glutamicum strain F, and the strain produces vanillate from ferulic acid and the enzyme converts it to protocatechiuc acid, a precursor of polymers and plastics and a constituent of food, fed-batch biotransformation Corynebacterium efficiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.82 additional information generation of recombinant Corynebacterium glutamicum strain F, overexpression of vanillate O-demethylase (VanAB) to increase the production of protocatechiuc acid from ferulic acid. The strain FVan produces vanillate from ferulic acid and the enzyme converts it to protocatechiuc acid, a precursor of polymers and plastics and a constituent of food, fed-batch biotransformation, protocatechiuc acid is secreted to the medium, overview Corynebacterium efficiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.82 vanillate + O2 + NADH + H+ Corynebacterium efficiens i.e. protocatechiuc acid 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?
1.14.13.82 vanillate + O2 + NADH + H+ Corynebacterium efficiens NBRC 100395 i.e. protocatechiuc acid 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.82 Corynebacterium efficiens Q8FRX4 AND Q8FRX3 vanillate O-demethylase oxygenase subunits A and B
-
1.14.13.82 Corynebacterium efficiens NBRC 100395 Q8FRX4 AND Q8FRX3 vanillate O-demethylase oxygenase subunits A and B
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.82 vanillate + O2 + NADH + H+ i.e. protocatechiuc acid Corynebacterium efficiens 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?
1.14.13.82 vanillate + O2 + NADH + H+ i.e. protocatechiuc acid Corynebacterium efficiens NBRC 100395 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.82 VAN
-
 Corynebacterium efficiens
1.14.13.82 vanillate O-demethylase
-
 Corynebacterium efficiens
1.14.13.82 vanillate O-demethylase oxygenase UniProt Corynebacterium efficiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.82 30
-
 in vivo assay at Corynebacterium efficiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.82 NADH
-
 Corynebacterium efficiens