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Literature summary extracted from

  • Kaczmarzyk, D.; Hudson, E.P.; Fulda, M.
    Arabidopsis acyl-acyl carrier protein synthetase AAE15 with medium chain fatty acid specificity is functional in cyanobacteria (2016), AMB Express, 6, doi: 10.1186/s13568-016-0178-z .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.20 gene AAE15 or At4g14070, recombinant expression of N-terminally His6-tagged enzyme, full-length or lacking the predicted plastidial targeting signal sequence, in Spodoptera frugiperda Sf9 cells via baculovirus transfection method, recombinant expression of Arabidopsis thaliana AAE15 in an AAS deletion mutant of Synechocystis sp. PCC 6803 resulting in changes in intracellular and extracellular free fatty acids pools in the cyanobacterial DELTAaas strain Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.20 additional information generation of a N-terminally truncated enzyme mutant with deleted predicted plastidial targeting signal sequence by removing the sequence encoding a predicted plastidial targeting peptide increases the expression level significantly Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.20 chloroplast the enzyme has a predicted plastidial targeting peptide Arabidopsis thaliana 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.20 Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.20 ATP + a long-chain fatty acid + an [acyl-carrier protein] Arabidopsis thaliana
-
 AMP + diphosphate + a long-chain acyl-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.20 Arabidopsis thaliana Q8W471
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.20 recombinant N-terminally His6-tagged enzyme, full-length or lacking the predicted plastidial targeting signal sequence, from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography, gel filtration, and dialysis. Removing the sequence encoding a predicted plastidial targeting peptide increases the expression level significantly, like DELTAaas, DELTAaas:AAE15 secretes large amounts of fatty acids into the culture medium. Expression of AAE15 in the DELTAaas strain does not affect fatty acid profiles and does not have a significant complementation effect Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.20 leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.20 0.0000667
-
 purified recombinant enzyme, pH 8.0, 37°C, with substrate decanoate Arabidopsis thaliana
6.2.1.20 0.0001027
-
 purified recombinant enzyme, pH 8.0, 37°C, with substrate laurate Arabidopsis thaliana
6.2.1.20 0.0001572
-
 purified recombinant enzyme, pH 8.0, 37°C, with substrate myristate Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.20 ATP + a long-chain fatty acid + an [acyl-carrier protein]
-
 Arabidopsis thaliana AMP + diphosphate + a long-chain acyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + decanoate + an [acyl-carrier protein]
-
 Arabidopsis thaliana AMP + diphosphate + long-chain decanoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + hexadecanoate + an [acyl-carrier protein] low activity Arabidopsis thaliana AMP + diphosphate + hexadecanoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + laurate + an [acyl-carrier protein]
-
 Arabidopsis thaliana AMP + diphosphate + lauroyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + myristate + an [acyl-carrier protein]
-
 Arabidopsis thaliana AMP + diphosphate + myristoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + octanoate + an [acyl-carrier protein] low activity Arabidopsis thaliana AMP + diphosphate + octanoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 additional information recombinant enzyme AAE15 has acyl-ACP synthetase activity in vitro with specificity for medium chain fatty acids, substrate specificity, overview. Low activity with fatty acids 18:0, 18:1, 18:2, and 18:3 Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.20 ? x * 79000, recombinant truncated enzyme lacking the predicted plastidial targeting signal sequence, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
6.2.1.20 AAE15
-
 Arabidopsis thaliana
6.2.1.20 AAS
-
 Arabidopsis thaliana
6.2.1.20 Acyl-ACP synthetase
-
 Arabidopsis thaliana
6.2.1.20 Acyl-acyl carrier protein synthetase
-
 Arabidopsis thaliana
6.2.1.20 At4g14070
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.20 37
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.20 8
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.20 ATP
-
 Arabidopsis thaliana