EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.11 | expression in Escherichia coli | Rhodococcus opacus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.11 | additional information | - |
isoform StyA2B constitutes dimers under reduced conditions | Rhodococcus opacus |
1.14.14.11 | additional information | - |
StyA1 is a dimer in its active form, gel filtration | Rhodococcus opacus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.11 | Rhodococcus opacus | A0A076JVU4 | styrene monooxygenase StyA | - |
1.14.14.11 | Rhodococcus opacus | C7ACG0 and C7ACG1 | i.e. oxygenase components StyA1 and StyA2B | - |
1.14.14.11 | Rhodococcus opacus | C7ACG1 | - |
- |
1.14.14.11 | Rhodococcus opacus 1CP | A0A076JVU4 | styrene monooxygenase StyA | - |
1.14.14.11 | Rhodococcus opacus 1CP | C7ACG0 and C7ACG1 | i.e. oxygenase components StyA1 and StyA2B | - |
1.14.14.11 | Rhodococcus opacus 1CP | C7ACG1 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.14.11 | 0.081 | - |
substrate styrene, presence of FAD reductase StyB originated from Pseudomonas fluorescens ST, pH 7.5, 30°C | Rhodococcus opacus |
1.14.14.11 | 0.087 | - |
substrate styrene, presence of FAD reductase StyB originated from Pseudomonas fluorescens ST, pH 7.5, 30°C | Rhodococcus opacus |
1.14.14.11 | 0.12 | - |
substrate styrene, presence of FAD reductase StyB originated from Pseudomonas fluorescens ST, pH 7.5, 30°C | Rhodococcus opacus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.11 | phenyl vinyl sulfide + FADH2 + O2 | - |
Rhodococcus opacus | (S)-phenyl vinyl sulfoxide + FAD + H2O | - |
? | |
1.14.14.11 | phenyl vinyl sulfide + FADH2 + O2 | - |
Rhodococcus opacus 1CP | (S)-phenyl vinyl sulfoxide + FAD + H2O | - |
? | |
1.14.14.11 | styrene + FADH2 + O2 | - |
Rhodococcus opacus | (S)-styrene oxide + FAD + H2O | - |
? | |
1.14.14.11 | styrene + FADH2 + O2 | - |
Rhodococcus opacus 1CP | (S)-styrene oxide + FAD + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.11 | dimer | enzyme is active as a heterodimer with flavin oxidoreductase StyB. 1 * 46580, StyA, plus 1 * 19053, StyB, calculated, 1 * 50000, StyA plus 1 * 21500, StyB, SDS-PAGE of recombinant His-tagged proteins | Rhodococcus opacus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.11 | StyA | - |
Rhodococcus opacus |
1.14.14.11 | StyA2B | - |
Rhodococcus opacus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.11 | FAD | incorporation of reduced FAD into StyA enzymes is attended with significant conformational rearrangements | Rhodococcus opacus |