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Literature summary extracted from

  • Nakazawa, H.; Kumagai, H.; Yamada, H.
    Aromatic L-amino acid decarboxylase from Micrococcus percitreus purification, crystallization and properties (1981), Agric. Biol. Chem., 45, 2543-2552 .
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.28 2.4
-
 L-tryptophan at pH 9.0 and 30°C Micrococcus percitreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.28 101000
-
 gel filtration Micrococcus percitreus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.28 L-tryptophan Micrococcus percitreus 100% activity tryptamine + CO2
-
 ?
4.1.1.28 L-tryptophan Micrococcus percitreus AJ 1065 100% activity tryptamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.28 Micrococcus percitreus
-
-
-
4.1.1.28 Micrococcus percitreus AJ 1065
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.28 ammonium sulfate precipitation, DEAE-Sephadex column chromatography, and Sephadex G-200 gel filtration Micrococcus percitreus

Storage Stability

EC Number Storage Stability Organism
4.1.1.28 5°C, 0.5 M potassium phosphate buffer pH 7.0 containing 20% (v/v) glycerol and 10 mM pyridoxal phosphate, more than 10 months, no loss of activity Micrococcus percitreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.28 2-chloro-L-tyrosine 77% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 2-methyl-L-tyrosine 75% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 3,4-Dihydroxy-L-phenylalanine 10% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 3,4-Dihydroxy-L-phenylalanine 10% activity compared to L-tryptophan Micrococcus percitreus AJ 1065 ?
-
 ?
4.1.1.28 3,5-dibromo-DL-tyrosine 72% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 3-chloro-L-tyrosine 81% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 3-Hydroxy-DL-kynurenine 8% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 3-Hydroxy-DL-kynurenine 8% activity compared to L-tryptophan Micrococcus percitreus AJ 1065 ?
-
 ?
4.1.1.28 3-methyl-L-tyrosine 80% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 5-fluoro-DL-tryptophan 75% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 5-hydroxy-L-tryptophan 24% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 5-methyl-DL-tryptophan 17% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 7-aza-DL-tryptophan 8% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 alpha-methyl-DL-phenylalanine 2% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 alpha-methyl-DL-tyrosine 1% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 L-2,4-dihydroxyphenylalanine 75% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 L-kynurenine 15% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 L-phenylalanine 120% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 L-tryptophan 100% activity Micrococcus percitreus tryptamine + CO2
-
 ?
4.1.1.28 L-tryptophan 100% activity Micrococcus percitreus AJ 1065 tryptamine + CO2
-
 ?
4.1.1.28 L-tyrosine 87% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 L-tyrosine 87% activity compared to L-tryptophan Micrococcus percitreus AJ 1065 ?
-
 ?
4.1.1.28 m-DL-tyrosine 5% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?
4.1.1.28 additional information no activity with 4-nitro-DL-phenylalanine, 3,5-dinitro-DL-tyrosine, 2,4,4-trihydroxyphenyl-L-alanine, alpha-methyl-3,4-dihydroxy-phenyl-L-alanine, 2-phenyl-DL-serine, 2-thiazole-DL-alanine, and L-histidine Micrococcus percitreus ?
-
 ?
4.1.1.28 additional information no activity with 4-nitro-DL-phenylalanine, 3,5-dinitro-DL-tyrosine, 2,4,4-trihydroxyphenyl-L-alanine, alpha-methyl-3,4-dihydroxy-phenyl-L-alanine, 2-phenyl-DL-serine, 2-thiazole-DL-alanine, and L-histidine Micrococcus percitreus AJ 1065 ?
-
 ?
4.1.1.28 o-DL-tyrosine 32% activity compared to L-tryptophan Micrococcus percitreus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.28 homodimer 2 * 48000, SDS-PAGE Micrococcus percitreus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.28 Aromatic L-amino acid decarboxylase
-
 Micrococcus percitreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.28 35 50 the purified enzyme is stable until 35°C for 60 min but rapidly inactivated at above 50°C Micrococcus percitreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.28 9
-
-
 Micrococcus percitreus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.28 6 7 the enzyme is stable for 60 min between pH 6.0 and 7.0 Micrococcus percitreus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.28 pyridoxal 5'-phosphate
-
 Micrococcus percitreus