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Literature summary extracted from

  • Ma, Q.; You, E.; Wang, J.; Wang, Y.; Ding, Z.
    Isolation and expression of CsFAD7 and CsFAD8, two genes encoding omega-3 fatty acid desaturase from Camellia sinensis (2014), Acta Physiol. Plant., 36, 2345-2352 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.35 gene fad7, DNA and amino acid sequence determination and analysis, sequence comparisons,quantitative real-time PCR enzyme expression analysis, phylogenetic analysis Camellia sinensis
1.14.19.35 gene fad8, DNA and amino acid sequence determination and analysis, sequence comparisons,quantitative real-time PCR enzyme expression analysis, phylogenetic analysis Camellia sinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.35 chloroplast
-
 Camellia sinensis 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.35 alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Camellia sinensis
-
 alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.35 Camellia sinensis L0GGK2 cv. Longjing43
-
1.14.19.35 Camellia sinensis S5GIT3 cv. Longjing43
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.35 leaf
-
 Camellia sinensis
-
1.14.19.35 additional information tissue-specific analysis of fatty acid patterns, overview. Enzyme FAD7 is detected in all vegetative tissues, and more abundant in the shoots and mature leaves, which are rich in chlorophyll, expression patterns Camellia sinensis
-
1.14.19.35 additional information tissue-specific analysis of fatty acid patterns, overview. Enzyme FAD8 is detected in all vegetative tissues, and more abundant in the shoots and mature leaves, which are rich in chlorophyll, expression patterns Camellia sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.35 alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Camellia sinensis alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.35 ? x * 50100, about, sequence calculation Camellia sinensis
1.14.19.35 ? x * 51500, about, sequence calculation Camellia sinensis

Synonyms

EC Number Synonyms Comment Organism
1.14.19.35 FAD7
-
 Camellia sinensis
1.14.19.35 FAD8
-
 Camellia sinensis
1.14.19.35 omega-3 fatty acid desaturase
-
 Camellia sinensis
1.14.19.35 plastidial omega-3 desaturase
-
 Camellia sinensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.35 Ferredoxin
-
 Camellia sinensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.19.35 Camellia sinensis sequence calculation
-
 8.1
1.14.19.35 Camellia sinensis sequence calculation
-
 8.9

Expression

EC Number Organism Comment Expression
1.14.19.35 Camellia sinensis at 4°C, the transcript level of CsFAD7 decreases significantly 1 h after the stress and then recovers. After 6 h, the expression level is of CsFAD7 remains at levels below the control (0 h) until 48 h, at -5°C, CsFAD7 decreases significantly and then remains stable until 48 h down
1.14.19.35 Camellia sinensis at 4°C, the transcript level of CsFAD8 decreases significantly 1 h after the stress and then recovers. After 6 h, the expression level of FAD8 is higher than the control at 24 h, at -5°C, CsFAD8 expression decreases significantly and then remains stable until 48 h down
1.14.19.35 Camellia sinensis wounding and abscisic acid induce the enzyme up
1.14.19.35 Camellia sinensis wounding and abscisic acid induce the enzyme rapidly, while the expression drops below control afterwards up

General Information

EC Number General Information Comment Organism
1.14.19.35 physiological function the plastidial omega-3-fatty acid desaturases CsFAD7 and CsFAD8 are both responsive to abiotic stress signals, but they may play very different roles during stress tolerance in tea plants Camellia sinensis