BRENDA - Enzyme Database

Molecular cloning and functional identification of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase from Tripterygium wilfordii

Cheng, Q.; Tong, Y.; Wang, Z.; Su, P.; Gao, W.; Huang, L.; Acta Pharm. Sin. B 7, 208-214 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.17.7.4
expressed in Escherichia coli DH5alpha cells
Tripterygium wilfordii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.7.4
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + reduced ferredoxin [iron-sulfur] cluster + H+
Tripterygium wilfordii
-
dimethylallyl diphosphate + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.17.7.4
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + reduced ferredoxin [iron-sulfur] cluster + H+
Tripterygium wilfordii
-
isopentenyl diphosphate + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.7.4
Tripterygium wilfordii
A0A088GJ08
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.7.4
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + reduced ferredoxin [iron-sulfur] cluster + H+
-
743969
Tripterygium wilfordii
dimethylallyl diphosphate + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.17.7.4
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + reduced ferredoxin [iron-sulfur] cluster + H+
-
743969
Tripterygium wilfordii
isopentenyl diphosphate + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.17.7.4
?
x * 52100, calculated from amino acid sequence
Tripterygium wilfordii
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.17.7.4
Tripterygium wilfordii
calculated from amino acid sequence
-
5.6
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.7.4
expressed in Escherichia coli DH5alpha cells
Tripterygium wilfordii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.7.4
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + reduced ferredoxin [iron-sulfur] cluster + H+
Tripterygium wilfordii
-
dimethylallyl diphosphate + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.17.7.4
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + reduced ferredoxin [iron-sulfur] cluster + H+
Tripterygium wilfordii
-
isopentenyl diphosphate + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.7.4
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + reduced ferredoxin [iron-sulfur] cluster + H+
-
743969
Tripterygium wilfordii
dimethylallyl diphosphate + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.17.7.4
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + reduced ferredoxin [iron-sulfur] cluster + H+
-
743969
Tripterygium wilfordii
isopentenyl diphosphate + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.7.4
?
x * 52100, calculated from amino acid sequence
Tripterygium wilfordii
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.17.7.4
Tripterygium wilfordii
calculated from amino acid sequence
-
5.6
Expression
EC Number
Organism
Commentary
Expression
1.17.7.4
Tripterygium wilfordii
enzyme expression is induced by methyl jasmonate reaching the highest level after 1 h
up
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.17.7.4
Tripterygium wilfordii
enzyme expression is induced by methyl jasmonate reaching the highest level after 1 h
up