Literature summary extracted from

Songsiriritthigul, C.; Suebka, S.; Chen, C.J.; Fuengfuloy, P.; Chuawong, P.
Crystal structure of the N-terminal anticodon-binding domain of the nondiscriminating aspartyl-tRNA synthetase from Helicobacter pylori (2017), Acta Crystallogr. Sect. F, 73, 62-69 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
6.1.1.12	expressed in Escherichia coli BL21(DE3) cells	Helicobacter pylori

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
6.1.1.12	hanging drop vapor diffusion method, using 7% (w/v) PEG 4000, 0.1 M ammonium sulfate, 0.1 M sodium citrate pH 5.6	Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6.1.1.12	ATP + L-aspartate + tRNAAsp	Helicobacter pylori	-	AMP + diphosphate + L-aspartyl-tRNAAsp	-	?
6.1.1.12	ATP + L-aspartate + tRNAAsp	Helicobacter pylori ATCC 700392	-	AMP + diphosphate + L-aspartyl-tRNAAsp	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.1.1.12	Helicobacter pylori	P56459	-	-
6.1.1.12	Helicobacter pylori ATCC 700392	P56459	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
6.1.1.12	Ni-NTA column chromatography, and Sephacryl S200 gel filtration	Helicobacter pylori

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.1.1.12	ATP + L-aspartate + tRNAAsp	-	Helicobacter pylori	AMP + diphosphate + L-aspartyl-tRNAAsp	-	?
6.1.1.12	ATP + L-aspartate + tRNAAsp	-	Helicobacter pylori ATCC 700392	AMP + diphosphate + L-aspartyl-tRNAAsp	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
6.1.1.12	Aspartyl-tRNA synthetase	-	Helicobacter pylori
6.1.1.12	AspRS	-	Helicobacter pylori

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
6.1.1.12	ATP	-	Helicobacter pylori

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Release 2023.1 (January 2023)