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Literature summary extracted from

  • Goyal, A.; Ahmed, S.; Sharma, K.; Gupta, V.; Bule, P.; Alves, V.D.; Fontes, C.M.; Najmudin, S.
    Molecular determinants of substrate specificity revealed by the structure of Clostridium thermocellum arabinofuranosidase 43A from glycosyl hydrolase family 43 subfamily 16 (2016), Acta crystallogr. Sect. D, 72, 1281-1289 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expressed in Escherichia coli BL21(DE3) pLysS cells Acetivibrio thermocellus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.55 vapor diffusion method, using either 0.1 M sodium acetate pH 4.5, 2 M ammonium sulfate or 0.1 M Tris pH 8.5, 20% (w/v) PEG 2000 monomethyl ether, 0.2 M trimethylamine N-oxide Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Acetivibrio thermocellus A3DEX4
-
-
3.2.1.55 Acetivibrio thermocellus DSM 1237 A3DEX4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 immobilized metal affinity column chromatography, and Superdex 75 gel filtration Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Acetivibrio thermocellus 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Acetivibrio thermocellus DSM 1237 4-nitrophenol + alpha-L-arabinofuranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 ? x * 36000, SDS-PAGE Acetivibrio thermocellus
3.2.1.55 ? x * 35860, calculated from amino acid sequence Acetivibrio thermocellus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 Abf43A
-
 Acetivibrio thermocellus
3.2.1.55 arabinofuranosidase 43A
-
 Acetivibrio thermocellus