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Literature summary extracted from

  • Sugiarto, G.; Lau, K.; Qu, J.; Li, Y.; Lim, S.; Mu, S.; Ames, J.B.; Fisher, A.J.; Chen, X.
    A sialyltransferase mutant with decreased donor hydrolysis and reduced sialidase activities for directly sialylating Lewisx (2012), ACS Chem. Biol., 7, 1232-1240 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.4.3.6 D141A the mutation results in 1000fold decrease in the efficiency of alpha2-3-sialyltransferase activity Pasteurella multocida
2.4.3.6 E271F/R313Y the mutant does not efficiently sialylate fucose-containing oligosaccharides such as Lex Pasteurella multocida
2.4.3.6 H311A the mutation results in 16fold decrease in the efficiency of alpha2-3-sialyltransferase activity Pasteurella multocida
2.4.3.6 M144D the mutant shows 20000fold decreased donor hydrolysis activity without significantly affecting its alpha2-3-sialylation activity when a poor fucose-containing acceptor substrate is used Pasteurella multocida
2.4.3.6 M144H the mutation results in 3fold decrease in the efficiency of alpha2-3-sialyltransferase activity Pasteurella multocida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.6 0.3
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144D, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 0.39
-
CMP-N-acetyl-beta-neuraminate wild type enzyme, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 0.4
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144H, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 0.44
-
CMP-N-acetyl-beta-neuraminate wild type enzyme, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 0.79
-
4-methylumbelliferyl beta-lactoside mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 0.81
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144H, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 1.4
-
CMP-N-acetyl-beta-neuraminate mutant enzyme D141A, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 1.4
-
4-methylumbelliferyl beta-lactoside wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 1.5
-
CMP-N-acetyl-beta-neuraminate wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 1.8
-
CMP-N-acetyl-beta-neuraminate mutant enzyme H311A, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 2.1
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144D, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 7.3
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 8.1
-
4-methylumbelliferyl beta-Lewis x mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 12
-
4-methylumbelliferyl beta-lactoside mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 13
-
4-methylumbelliferyl beta-Lewis x mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 13
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 17
-
4-methylumbelliferyl beta-Lewis x wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.6 Pasteurella multocida Q15KI8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.6
-
Pasteurella multocida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.6 CMP-N-acetyl-beta-neuraminate + 4-methylumbelliferyl beta-lactoside
-
Pasteurella multocida CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-Glc
-
?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + 4-methylumbelliferyl beta-Lewis x
-
Pasteurella multocida CMP + 4-methylumbelliferyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc
-
?
2.4.3.6 CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-[alpha-D-Fuc-(1->3)]-beta-D-GlcNAc-[protein] i.e. Lewis x epitope Pasteurella multocida CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-[alpha-D-Fuc-(1->3)]-beta-D-GlcNAc-[protein]
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.3.6 PmST1
-
Pasteurella multocida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.3.6 0.025
-
CMP-N-acetyl-beta-neuraminate mutant enzyme D141A, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 0.55
-
CMP-N-acetyl-beta-neuraminate wild type enzyme, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 0.59
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144D, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 0.93
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144H, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 1.9
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144D, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 2.1
-
CMP-N-acetyl-beta-neuraminate mutant enzyme H311A, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 4
-
4-methylumbelliferyl beta-Lewis x mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 6.5
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 6.7
-
4-methylumbelliferyl beta-Lewis x wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 8.4
-
4-methylumbelliferyl beta-Lewis x mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 21
-
4-methylumbelliferyl beta-lactoside mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 21
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144H, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 22
-
4-methylumbelliferyl beta-lactoside mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 27
-
CMP-N-acetyl-beta-neuraminate wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 32
-
CMP-N-acetyl-beta-neuraminate wild type enzyme, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 47
-
4-methylumbelliferyl beta-lactoside wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 71
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.3.6 0.018
-
CMP-N-acetyl-beta-neuraminate mutant enzyme D141A, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 0.28
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144D, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 0.32
-
4-methylumbelliferyl beta-Lewis x mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 0.38
-
4-methylumbelliferyl beta-Lewis x wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 0.89
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 1
-
4-methylumbelliferyl beta-Lewis x mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 1.1
-
CMP-N-acetyl-beta-neuraminate mutant enzyme H311A, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 1.4
-
CMP-N-acetyl-beta-neuraminate wild type enzyme, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 1.9
-
4-methylumbelliferyl beta-lactoside mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 2.2
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144H, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 5.5
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 6.1
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144D, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 18
-
CMP-N-acetyl-beta-neuraminate wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 27
-
4-methylumbelliferyl beta-lactoside mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 27
-
CMP-N-acetyl-beta-neuraminate mutant enzyme M144H, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 34
-
4-methylumbelliferyl beta-lactoside wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.6 73
-
CMP-N-acetyl-beta-neuraminate wild type enzyme, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C Pasteurella multocida