EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.3.6 | D141A | the mutation results in 1000fold decrease in the efficiency of alpha2-3-sialyltransferase activity | Pasteurella multocida |
2.4.3.6 | E271F/R313Y | the mutant does not efficiently sialylate fucose-containing oligosaccharides such as Lex | Pasteurella multocida |
2.4.3.6 | H311A | the mutation results in 16fold decrease in the efficiency of alpha2-3-sialyltransferase activity | Pasteurella multocida |
2.4.3.6 | M144D | the mutant shows 20000fold decreased donor hydrolysis activity without significantly affecting its alpha2-3-sialylation activity when a poor fucose-containing acceptor substrate is used | Pasteurella multocida |
2.4.3.6 | M144H | the mutation results in 3fold decrease in the efficiency of alpha2-3-sialyltransferase activity | Pasteurella multocida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.6 | 0.3 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144D, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 0.39 | - |
CMP-N-acetyl-beta-neuraminate | wild type enzyme, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 0.4 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144H, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 0.44 | - |
CMP-N-acetyl-beta-neuraminate | wild type enzyme, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 0.79 | - |
4-methylumbelliferyl beta-lactoside | mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 0.81 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144H, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 1.4 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme D141A, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 1.4 | - |
4-methylumbelliferyl beta-lactoside | wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 1.5 | - |
CMP-N-acetyl-beta-neuraminate | wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 1.8 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme H311A, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 2.1 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144D, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 7.3 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 8.1 | - |
4-methylumbelliferyl beta-Lewis x | mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 12 | - |
4-methylumbelliferyl beta-lactoside | mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 13 | - |
4-methylumbelliferyl beta-Lewis x | mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 13 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 17 | - |
4-methylumbelliferyl beta-Lewis x | wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.3.6 | Pasteurella multocida | Q15KI8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.3.6 | - |
Pasteurella multocida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.6 | CMP-N-acetyl-beta-neuraminate + 4-methylumbelliferyl beta-lactoside | - |
Pasteurella multocida | CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-Glc | - |
? | |
2.4.3.6 | CMP-N-acetyl-beta-neuraminate + 4-methylumbelliferyl beta-Lewis x | - |
Pasteurella multocida | CMP + 4-methylumbelliferyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc | - |
? | |
2.4.3.6 | CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->4)-[alpha-D-Fuc-(1->3)]-beta-D-GlcNAc-[protein] | i.e. Lewis x epitope | Pasteurella multocida | CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-[alpha-D-Fuc-(1->3)]-beta-D-GlcNAc-[protein] | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.3.6 | PmST1 | - |
Pasteurella multocida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.6 | 0.025 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme D141A, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 0.55 | - |
CMP-N-acetyl-beta-neuraminate | wild type enzyme, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 0.59 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144D, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 0.93 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144H, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 1.9 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144D, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 2.1 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme H311A, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 4 | - |
4-methylumbelliferyl beta-Lewis x | mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 6.5 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 6.7 | - |
4-methylumbelliferyl beta-Lewis x | wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 8.4 | - |
4-methylumbelliferyl beta-Lewis x | mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 21 | - |
4-methylumbelliferyl beta-lactoside | mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 21 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144H, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 22 | - |
4-methylumbelliferyl beta-lactoside | mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 27 | - |
CMP-N-acetyl-beta-neuraminate | wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 32 | - |
CMP-N-acetyl-beta-neuraminate | wild type enzyme, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 47 | - |
4-methylumbelliferyl beta-lactoside | wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 71 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.6 | 0.018 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme D141A, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 0.28 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144D, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 0.32 | - |
4-methylumbelliferyl beta-Lewis x | mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 0.38 | - |
4-methylumbelliferyl beta-Lewis x | wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 0.89 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 1 | - |
4-methylumbelliferyl beta-Lewis x | mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 1.1 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme H311A, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 1.4 | - |
CMP-N-acetyl-beta-neuraminate | wild type enzyme, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 1.9 | - |
4-methylumbelliferyl beta-lactoside | mutant enzyme M144D, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 2.2 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144H, using 4-methylumbelliferyl beta-Lewis x as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 5.5 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 6.1 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144D, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 18 | - |
CMP-N-acetyl-beta-neuraminate | wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 27 | - |
4-methylumbelliferyl beta-lactoside | mutant enzyme M144H, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 27 | - |
CMP-N-acetyl-beta-neuraminate | mutant enzyme M144H, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 34 | - |
4-methylumbelliferyl beta-lactoside | wild type enzyme, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida | |
2.4.3.6 | 73 | - |
CMP-N-acetyl-beta-neuraminate | wild type enzyme, using 4-methylumbelliferyl beta-lactoside as cosubstrate, in Tris-HCl buffer (200 mM, pH 8.5), at 37°C | Pasteurella multocida |