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Literature summary extracted from

  • Zallot, R.; Ross, R.; Chen, W.H.; Bruner, S.D.; Limbach, P.A.; de Crecy-Lagard, V.
    Identification of a novel epoxyqueuosine reductase family by comparative genomics (2017), ACS Chem. Biol., 12, 844-851 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.99.6 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL, Rosetta 2(DE3) pLysS, and OverExpress C41(DE3) cells Lacticaseibacillus casei
1.17.99.6 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL, Rosetta 2(DE3) pLysS, and OverExpress C41(DE3) cells Ligilactobacillus salivarius
1.17.99.6 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL, Rosetta 2(DE3) pLysS, and OverExpress C41(DE3) cells Acinetobacter baylyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.99.6 queuosine34 in tRNA + acceptor + H2O Lacticaseibacillus casei
-
 epoxyqueuosine34 in tRNA + reduced acceptor
-
 ?
1.17.99.6 queuosine34 in tRNA + acceptor + H2O Ligilactobacillus salivarius
-
 epoxyqueuosine34 in tRNA + reduced acceptor
-
 ?
1.17.99.6 queuosine34 in tRNA + acceptor + H2O Acinetobacter baylyi
-
 epoxyqueuosine34 in tRNA + reduced acceptor
-
 ?
1.17.99.6 queuosine34 in tRNA + acceptor + H2O Lacticaseibacillus casei ATCC 334
-
 epoxyqueuosine34 in tRNA + reduced acceptor
-
 ?
1.17.99.6 queuosine34 in tRNA + acceptor + H2O Ligilactobacillus salivarius ATCC 11741
-
 epoxyqueuosine34 in tRNA + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.99.6 Acinetobacter baylyi Q6FAK7
-
-
1.17.99.6 Lacticaseibacillus casei
-
-
-
1.17.99.6 Lacticaseibacillus casei ATCC 334
-
-
-
1.17.99.6 Ligilactobacillus salivarius
-
-
-
1.17.99.6 Ligilactobacillus salivarius ATCC 11741
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.99.6 Ni-NTA column chromatography and Superdex 200 gel filtration Lacticaseibacillus casei
1.17.99.6 Ni-NTA column chromatography and Superdex 200 gel filtration Ligilactobacillus salivarius
1.17.99.6 Ni-NTA column chromatography and Superdex 200 gel filtration Acinetobacter baylyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.99.6 queuosine34 in tRNA + acceptor + H2O
-
 Lacticaseibacillus casei epoxyqueuosine34 in tRNA + reduced acceptor
-
 ?
1.17.99.6 queuosine34 in tRNA + acceptor + H2O
-
 Ligilactobacillus salivarius epoxyqueuosine34 in tRNA + reduced acceptor
-
 ?
1.17.99.6 queuosine34 in tRNA + acceptor + H2O
-
 Acinetobacter baylyi epoxyqueuosine34 in tRNA + reduced acceptor
-
 ?
1.17.99.6 queuosine34 in tRNA + acceptor + H2O
-
 Lacticaseibacillus casei ATCC 334 epoxyqueuosine34 in tRNA + reduced acceptor
-
 ?
1.17.99.6 queuosine34 in tRNA + acceptor + H2O
-
 Ligilactobacillus salivarius ATCC 11741 epoxyqueuosine34 in tRNA + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.99.6 duf 208
-
 Lacticaseibacillus casei
1.17.99.6 duf 208
-
 Ligilactobacillus salivarius
1.17.99.6 duf 208
-
 Acinetobacter baylyi
1.17.99.6 oQ reductase
-
 Lacticaseibacillus casei
1.17.99.6 oQ reductase
-
 Ligilactobacillus salivarius
1.17.99.6 oQ reductase
-
 Acinetobacter baylyi
1.17.99.6 QueG
-
 Lacticaseibacillus casei
1.17.99.6 QueG
-
 Acinetobacter baylyi
1.17.99.6 queH
-
 Lacticaseibacillus casei
1.17.99.6 queH
-
 Acinetobacter baylyi