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Literature summary extracted from

  • Purka, P.; Ihsanawat, I.; Natalia, D.; Syah, Y.; Retnoningrum, D.; Kusuma, H.
    Mutation of katG in a clinical isolate of Mycobacterium tuberculosis Effects on catalase-peroxidase for isoniazid activation (2016), Ukr. Biochem. J., 88, 71-81 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.21 N238S the catalytic efficiency (Kcat/KM) of the mutant decreases to 41 and 52% for catalase and peroxidase, respectively, compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 0.25
-
 o-Dianisidine wild type enzyme, at pH 4.5 and 30°C Mycobacterium tuberculosis
1.11.1.21 0.35
-
 o-Dianisidine mutant enzyme N238S, at pH 4.5 and 30°C Mycobacterium tuberculosis
1.11.1.21 3.16
-
 H2O2 wild type enzyme, at pH 7.0 and 30°C Mycobacterium tuberculosis
1.11.1.21 4.44
-
 H2O2 mutant enzyme N238S, at pH 7.0 and 30°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Mycobacterium tuberculosis P9WIE5
-
-
1.11.1.21 Mycobacterium tuberculosis H37Rv P9WIE5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.21 HisTrap column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2 H2O2
-
 Mycobacterium tuberculosis O2 + H2O
-
 ?
1.11.1.21 2 H2O2
-
 Mycobacterium tuberculosis H37Rv O2 + H2O
-
 ?
1.11.1.21 isoniazid + phenol + H2O2
-
 Mycobacterium tuberculosis benzoquinone + ?
-
 ?
1.11.1.21 isoniazid + phenol + H2O2
-
 Mycobacterium tuberculosis H37Rv benzoquinone + ?
-
 ?
1.11.1.21 o-dianisidine + tert-butyl hydroperoxide + H2O2
-
 Mycobacterium tuberculosis o-dianisidin quinone diimine + ?
-
 ?
1.11.1.21 o-dianisidine + tert-butyl hydroperoxide + H2O2
-
 Mycobacterium tuberculosis H37Rv o-dianisidin quinone diimine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.21 ? x * 80000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 KatG
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.21 33.3
-
 o-Dianisidine mutant enzyme N238S, at pH 4.5 and 30°C Mycobacterium tuberculosis
1.11.1.21 49
-
 o-Dianisidine wild type enzyme, at pH 4.5 and 30°C Mycobacterium tuberculosis
1.11.1.21 227.5
-
 H2O2 mutant enzyme N238S, at pH 7.0 and 30°C Mycobacterium tuberculosis
1.11.1.21 272.3
-
 H2O2 wild type enzyme, at pH 7.0 and 30°C Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.21 51.2
-
 H2O2 mutant enzyme N238S, at pH 7.0 and 30°C Mycobacterium tuberculosis
1.11.1.21 86.2
-
 H2O2 wild type enzyme, at pH 7.0 and 30°C Mycobacterium tuberculosis
1.11.1.21 96
-
 o-Dianisidine mutant enzyme N238S, at pH 4.5 and 30°C Mycobacterium tuberculosis
1.11.1.21 199
-
 o-Dianisidine wild type enzyme, at pH 4.5 and 30°C Mycobacterium tuberculosis