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Literature summary extracted from

  • Khare, D.; Hale, W.; Tripathi, A.; Gu, L.; Sherman, D.; Gerwick, W.; Hakansson, K.; Smith, J.
    Structural basis for cyclopropanation by a unique enoyl-acyl carrier protein reductase (2015), Structure, 23, 2213-2223 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.B14
-
 Lyngbya majuscula
1.3.1.104
-
 Lyngbya majuscula

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.B14
-
 Lyngbya majuscula

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.104 D273A about 55% of wild-type activity Lyngbya majuscula
1.3.1.104 D273N about 70% of wild-type activity Lyngbya majuscula
1.3.1.104 K251A activity similar to wild-type Lyngbya majuscula
1.3.1.104 K251R activity similar to wild-type Lyngbya majuscula
1.3.1.104 additional information replacement of amino acids 55-66 by CurF enoyl reductase cyclopropanase loop leads to gain-of-function activity as a cyclopropanase. With substrate 3-chloromethyl-crotonyl-[acyl-carrier-protein], the formation of both the reduced product and also the cyclopropanated productis detected at levels of about one-quarter of the total product Lyngbya majuscula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.B14 (2E,4E,6E,10Z)-4-methyl-11-[2-[(1R,2S)-2-methylcyclopropyl]-4,5-dihydro-1,3-thiazol-4-yl]undeca-2,4,6,10-tetraenoyl-[acyl-carrier-protein] + NADPH + H+ Lyngbya majuscula
-
 (2E,4E,6E,10Z)-4-methyl-11-[2-[(1R,2S)-2-methylcyclopropyl]-4,5-dihydro-1,3-thiazol-4-yl]undeca-4,6,10-trienoyl-[acyl-carrier-protein] + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.B14 Lyngbya majuscula Q6DNE2
-
-
1.3.1.104 Lyngbya majuscula Q6E7K0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.B14 (2E,4E,6E,10Z)-4-methyl-11-[2-[(1R,2S)-2-methylcyclopropyl]-4,5-dihydro-1,3-thiazol-4-yl]undeca-2,4,6,10-tetraenoyl-[acyl-carrier-protein] + NADPH + H+
-
 Lyngbya majuscula (2E,4E,6E,10Z)-4-methyl-11-[2-[(1R,2S)-2-methylcyclopropyl]-4,5-dihydro-1,3-thiazol-4-yl]undeca-4,6,10-trienoyl-[acyl-carrier-protein] + NADP+
-
 ?
1.3.1.B14 crotonyl-CoA + NADPH + H+
-
 Lyngbya majuscula butanoyl-CoA + NADP+
-
 ?
1.3.1.104 crotonyl-CoA + NADPH + H+
-
 Lyngbya majuscula butanoyl-CoA + NADP+
-
 ?
1.3.1.104 gama-chloro-beta-methylcrotonyl-[acyl-carrier-protein] + NADPH + H+
-
 Lyngbya majuscula gama-chloro-beta-methylbutanoyl-[acyl-carrier-protein] + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.B14 curacin enoyl reductase
-
 Lyngbya majuscula
1.3.1.B14 CurK
-
 Lyngbya majuscula
1.3.1.104 JamJ
-
 Lyngbya majuscula

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.B14 NADPH loops that contact NADPH show flexibility Lyngbya majuscula

General Information

EC Number General Information Comment Organism
1.3.1.B14 physiological function enzyme is involved in biosynthesis of anticancer agent curacin A Lyngbya majuscula