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Literature summary extracted from

  • Min, K.; Gong, G.; Woo, H.M.; Kim, Y.; Um, Y.
    A dye-decolorizing peroxidase from Bacillus subtilis exhibiting substrate-dependent optimum temperature for dyes and beta-ether lignin dimer (2015), Sci. Rep., 5, 8245 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.19 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.19 Bacillus subtilis
-
-
-
1.11.1.19 Bacillus subtilis KCTC2023
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.19 Ni-NTA column chromatography, and Superdex 200 gel filtration Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.19 0.086
-
 with veratryl glycerol-beta-guaiacyl ether, at pH 3.0 and 50°C Bacillus subtilis
1.11.1.19 0.13
-
 with veratryl alcohol, at pH 3.0 and 50°C Bacillus subtilis
1.11.1.19 11.55
-
 with Reactive Blue 19, at pH 3.0 and 30°C Bacillus subtilis
1.11.1.19 17.65
-
 with Reactive Black 5, at pH 3.0 and 30°C Bacillus subtilis
1.11.1.19 66.8
-
 with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), at pH 3.0 and 50°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Bacillus subtilis ?
-
 ?
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Bacillus subtilis KCTC2023 ?
-
 ?
1.11.1.19 2,6-dimethoxyphenol + H2O2 + H+
-
 Bacillus subtilis ?
-
 ?
1.11.1.19 2,6-dimethoxyphenol + H2O2 + H+
-
 Bacillus subtilis KCTC2023 ?
-
 ?
1.11.1.19 Reactive Black 5 + H2O2 + H+
-
 Bacillus subtilis ?
-
 ?
1.11.1.19 Reactive Black 5 + H2O2 + H+
-
 Bacillus subtilis KCTC2023 ?
-
 ?
1.11.1.19 Reactive Blue 19 + H2O2 + H+
-
 Bacillus subtilis ?
-
 ?
1.11.1.19 Reactive Blue 19 + H2O2 + H+
-
 Bacillus subtilis KCTC2023 ?
-
 ?
1.11.1.19 veratryl alcohol + H2O2 + H+
-
 Bacillus subtilis ?
-
 ?
1.11.1.19 veratryl alcohol + H2O2 + H+
-
 Bacillus subtilis KCTC2023 ?
-
 ?
1.11.1.19 veratryl glycerol-beta-guaiacyl ether + H2O2 + H+
-
 Bacillus subtilis veratryl aldehyde + guaiacol + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.19 ? x * 48000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.11.1.19 dye-decolorizing peroxidase
-
 Bacillus subtilis
1.11.1.19 DyP
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.19 30
-
 optimum temperature for dyes Bacillus subtilis
1.11.1.19 50
-
 optimum temperature for high redox potential substrates Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.19 heme
-
 Bacillus subtilis