EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.2 | expressed in Escherichia coli BL21(DE3) cells | uncultured bacterium |
EC Number | General Stability | Organism |
---|---|---|
1.13.11.2 | the two isoforms C23O1 and C23O2 are both highly active over a broad range of NaCl concentration, even as high as 30% (w/v) salinity, while C23O1 presents a little higher activity than C23O2 | uncultured bacterium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.2 | Al3+ | the enzyme activity increases in the presence of Al3+ | uncultured bacterium | |
1.13.11.2 | Cu2+ | the enzyme activity increases in the presence of Cu2+ | uncultured bacterium | |
1.13.11.2 | Fe2+ | the enzyme activity increases in the presence of Fe2+ (isoform C23O1 shows 265.65% activity at 5 mM) | uncultured bacterium | |
1.13.11.2 | Fe3+ | the enzyme activity increases in the presence of Fe3+ (isoform C23O2 shows 377.37% activity at 5 mM) | uncultured bacterium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.2 | uncultured bacterium | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.2 | Ni-NTA column chromatography | uncultured bacterium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.2 | 3-methylcatechol + O2 | isoform C23O1 shows 49.5% activity compared to catechol, isoform C23O2 shows 239.2% activity compared to catechol | uncultured bacterium | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
1.13.11.2 | 4-chlorocatechol + O2 | isoform C23O1 shows 50.8% activity compared to catechol, isoform C23O2 shows 226.4% activity compared to catechol | uncultured bacterium | 4-chloro-2-hydroxymuconate semialdehyde | - |
? | |
1.13.11.2 | 4-methylcatechol + O2 | best substrate for isoform C23O2 (280.7% activity compared to catechol), while isoform C23O1 shows 87.4% activity compared to catechol | uncultured bacterium | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
1.13.11.2 | catechol + O2 | best substrate for isoform C23O1 (100% activity) | uncultured bacterium | 2-hydroxymuconate-6-semialdehyde | - |
? | |
1.13.11.2 | additional information | the two isoforms cannot oxidize 1, 2-dihydroxynaphthalene | uncultured bacterium | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.2 | ? | x * 35000, SDS-PAGE | uncultured bacterium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.2 | C23O1 | isoform | uncultured bacterium |
1.13.11.2 | C23O2 | isoform | uncultured bacterium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.2 | 40 | - |
isoform C23O1 | uncultured bacterium |
1.13.11.2 | 60 | - |
isoform C23O2 | uncultured bacterium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.2 | 4 | 30 | the two isoforms C23O1 and C23O2 are both highly stable in the temperature range of 4-30°C for 24 h. Their activities are kept best at 10°C and the residual activities are about 80% at 24 h, followed by 20°C with approximately 60% residual activies at 24 h. Their enzyme activities decline rapidly as the temperature increases above 50°C | uncultured bacterium |