Literature summary extracted from

Huang, L.; Hu, H.; Tang, H.; Liu, Y.; Xu, P.; Shi, J.; Lin, K.; Luo, Q.; Cui, C.
Identification and characterization of a novel gentisate 1,2-dioxygenase gene from a halophilic Martelella strain (2015), Sci. Rep., 5, 14307 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.13.11.4	expressed in Escherichia coli BL21(DE3) cells	Martelella sp. AD-3

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.13.11.4	H155A	inactive	Martelella sp. AD-3
1.13.11.4	H157A	inactive	Martelella sp. AD-3
1.13.11.4	H167A	inactive	Martelella sp. AD-3
1.13.11.4	H169A	inactive	Martelella sp. AD-3

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.13.11.4	Co2+	the addition of 0.005 mM Co2+ does inhibit enzyme activity to 53%	Martelella sp. AD-3

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.13.11.4	0.0264	-	2,5-Dihydroxybenzoate	at pH 7.0 and 23°C	Martelella sp. AD-3

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.13.11.4	Cu2+	activates	Martelella sp. AD-3
1.13.11.4	Fe2+	activates	Martelella sp. AD-3
1.13.11.4	Mn2+	activates	Martelella sp. AD-3
1.13.11.4	additional information	Ca2+, Ni2+, Mg2+, and Zn2+ have no impact on enzyme activity	Martelella sp. AD-3

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.13.11.4	120000	-	gel filtration	Martelella sp. AD-3

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.13.11.4	2,5-dihydroxybenzoate + O2	Martelella sp. AD-3	-	maleylpyruvate	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.13.11.4	Martelella sp. AD-3	A0A127CMI7	-	-

Storage Stability

EC Number	Storage Stability	Organism
1.13.11.4	-20°C, purified enzyme, 98 h, complete loss of activity	Martelella sp. AD-3

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.13.11.4	2,5-dihydroxybenzoate + O2	-	Martelella sp. AD-3	maleylpyruvate	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.13.11.4	homotrimer	3 * 38000, SDS-PAGE	Martelella sp. AD-3

Synonyms

EC Number	Synonyms	Comment	Organism
1.13.11.4	GDO	-	Martelella sp. AD-3

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.13.11.4	30	-	-	Martelella sp. AD-3

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.13.11.4	161.29	-	2,5-Dihydroxybenzoate	at pH 7.0 and 23°C	Martelella sp. AD-3

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.13.11.4	7	-	-	Martelella sp. AD-3

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Release 2023.1 (January 2023)