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Literature summary extracted from

  • Song, J.; Yu, Y.; Xing, R.; Guo, X.; Liu, D.; Wei, J.; Song, H.
    Unglycosylated recombinant human glutathione peroxidase 3 mutant from Escherichia coli is active as a monomer (2014), Sci. Rep., 4, 6698 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.9 expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.9 molecular modeling of mutant with all Cys residues changed to Ser Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.9 additional information expression of a recombinant human Gpx3 mutant with all Cys residues changed to Ser from a Cys auxotrophic strain of E. coli. Although lacking post-translational modification, the mutant still retains the ability to reduce H2O2 and 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine. Contrary to wild-type, the mutant exists as a monomer in solution Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.9 Homo sapiens P22352
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.9 8.5
-
 mutant with all Cys residues changed to Ser, substrate 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine, pH 7.4, 37°C Homo sapiens
1.11.1.9 25
-
 mutant with all Cys residues changed to Ser, substrate H2O2, pH 7.4, 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.9 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine + 2 GSH
-
 Homo sapiens 1-palmitoyl-2-(13-hydroxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine + GSSG + H2O
-
 ?
1.11.1.9 2 glutathione + H2O2
-
 Homo sapiens glutathione disulfide + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.9 monomer 1 * 26000, SDS-PAGE, recombinant mutant with all Cys residues changed to Ser Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.11.1.9 GPX3
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.9 45
-
 mutant with all Cys residues changed to Ser Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.11.1.9 55
-
 almost completely inactive Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.9 8.9
-
 mutant with all Cys residues changed to Ser Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.9 7.4 8.9
-
 Homo sapiens
1.11.1.9 9.8
-
 93% loss of activity Homo sapiens