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Literature summary extracted from

  • Shoji, M.; Sato, K.; Yukitake, H.; Naito, M.; Nakayama, K.
    Involvement of the Wbp pathway in the biosynthesis of Porphyromonas gingivalis lipopolysaccharide with anionic polysaccharide (2014), Sci. Rep., 4, 5056 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.335 gene wbpB or PGN_0168, from mutant strain HG66, DNA and amino acid sequence determination and analysis, complementation of the wbpB-defective mutant strain by expression of wild-type gene wbpB Porphyromonas gingivalis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.335 additional information detection nonsense mutation in the gene wbpB from strain HG66 by sequencing. Porphyromonas gingivalis strain HG66 lacks lipopolysaccharide A-LPS. Introduction of a wild-type wbpB gene into strain HG66 restores formation of A-LPS. Presence of a nonsense mutation in the gene, the porR mutation. The porR mutant possesses O-LPS, but lacks A-LPS Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.335 UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O Porphyromonas gingivalis
-
 UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+
-
 ?
1.1.1.335 UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O Porphyromonas gingivalis DSM 20709
-
 UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.335 Porphyromonas gingivalis B2RH42
-
-
1.1.1.335 Porphyromonas gingivalis DSM 20709 B2RH42
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.335 UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O
-
 Porphyromonas gingivalis UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+
-
 ?
1.1.1.335 UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O
-
 Porphyromonas gingivalis DSM 20709 UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.335 PGN_0168
-
 Porphyromonas gingivalis
1.1.1.335 WbpB
-
 Porphyromonas gingivalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.335 NAD+
-
 Porphyromonas gingivalis

General Information

EC Number General Information Comment Organism
1.1.1.335 malfunction mutants porR and porT are involved in the pigmentation phenotype in Porphyromonas gingivalis. Porphyromonas gingivalis has two different lipopolysaccharide (LPS) molecules, O-LPS and A-LPS. Porphyromonas gingivalis strain HG66 lacks lipopolysaccharide A-LPS. Introduction of a wild-type wbpB gene into strain HG66 restores formation of A-LPS. Sequencing of the wbpB gene from strain HG66 has revealed the presence of a nonsense mutation in the gene, the porR mutation. The porR mutant possesses O-LPS, but lacks A-LPS Porphyromonas gingivalis
1.1.1.335 physiological function A-LPS consists of a lipid A-core and anionic polysaccharide (APS) repeating units containing phosphorylated branched mannan. The PorR, WbpB and UgdA proteins are predicted to participate in the initial synthesis of structural sugar(s) in the anionic polysaccharide Porphyromonas gingivalis