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Literature summary extracted from
- A novel twist on molecular interactions between thioredoxin and nicotinamide adenine dinucleotide phosphate-dependent thioredoxin reductase (2014), Proteins, 82, 607-619 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | - |
Arabidopsis thaliana |
| 1.8.1.9 | - |
Hordeum vulgare subsp. vulgare |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | molecular modelling of the complex with thioredoxin | Hordeum vulgare subsp. vulgare |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | C145S | active site cysteine residue. Kinetic data | Hordeum vulgare subsp. vulgare |
| 1.8.1.9 | C148S | residue Cys148 probably performs an initial nucleophilic attack on the active site disulfide in thioredoxin disulfide. Kinetic data | Hordeum vulgare subsp. vulgare |
| 1.8.1.9 | Delta42-47 | FAD-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| 1.8.1.9 | G222D/A223G/G224E | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| 1.8.1.9 | G225R/G226D | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| 1.8.1.9 | G225R/G226D/P227V | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| 1.8.1.9 | M43A | mutant in a loop of the FAD-binding domain, strongly affects the interaction with thioredoxin. Kinetic data | Hordeum vulgare subsp. vulgare |
| 1.8.1.9 | N139A | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| 1.8.1.9 | N45A/D46A | FAD-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| 1.8.1.9 | R140A | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| 1.8.1.9 | W42A | mutant in a loop of the FAD-binding domain, strongly affects the interaction with thioredoxin. Kinetic data | Hordeum vulgare subsp. vulgare |
| 1.8.1.9 | W42A/M43A | mutant in a loop of the FAD-binding domain, strongly affects the interaction with thioredoxin | Hordeum vulgare subsp. vulgare |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 0.00041 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.00048 | - |
Arabidopsis thaliana thioredoxin 3 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.8.1.9 | 0.00052 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.00057 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.00067 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.00068 | - |
Hordeum vulgare thioredoxin 2 | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.00073 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.0008 | - |
Arabidopsis thaliana thioredoxin 3 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.00105 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.00117 | - |
Hordeum vulgare thioredoxin 2 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.00123 | - |
Hordeum vulgare thioredoxin 1 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.00128 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.0014 | - |
Hordeum vulgare thioredoxin 2 mutant G57P | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.00146 | - |
Hordeum vulgare thioredoxin 2 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.0016 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.0022 | - |
Hordeum vulgare thioredoxin 2 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.0024 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.00343 | - |
Hordeum vulgare thioredoxin 2 | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.0042 | - |
Arabidopsis thaliana thioredoxin 3 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.0141 | - |
Arabidopsis thaliana thioredoxin 3 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.0155 | - |
Hordeum vulgare thioredoxin 2 mutant I51G | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.016 | - |
Hordeum vulgare thioredoxin 2 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.0305 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A/M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.0457 | - |
Hordeum vulgare thioredoxin 2 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.0543 | - |
Arabidopsis thaliana thioredoxin 3 | mutant Delta42-47, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 0.06 | - |
Hordeum vulgare thioredoxin 2 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.8.1.9 | 0.149 | - |
Escherichia coli thioredoxin | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.1.9 | mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.9 | Arabidopsis thaliana | Q39243 | - |
- |
| 1.8.1.9 | Hordeum vulgare subsp. vulgare | A9LN30 | isoform Ntr2 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.9 | Arabidopsis thaliana thioredoxin 3 + NADP+ | - |
Arabidopsis thaliana | Arabidopsis thaliana thioredoxin 3 disulfide + NADPH + H+ | - |
? |  |
| 1.8.1.9 | Arabidopsis thaliana thioredoxin 3 + NADP+ | - |
Hordeum vulgare subsp. vulgare | Arabidopsis thaliana thioredoxin 3 disulfide + NADPH + H+ | - |
? | |
| 1.8.1.9 | Escherichia coli thioredoxin + NADP+ | - |
Arabidopsis thaliana | Escherichia coli thioredoxin disulfide + NADPH + H+ | - |
? | |
| 1.8.1.9 | Escherichia coli thioredoxin + NADP+ | - |
Hordeum vulgare subsp. vulgare | Escherichia coli thioredoxin disulfide + NADPH + H+ | - |
? | |
| 1.8.1.9 | Hordeum vulgare thioredoxin 1 + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 1 disulfide + NADPH + H+ | - |
? | |
| 1.8.1.9 | Hordeum vulgare thioredoxin 2 + NADP+ | - |
Arabidopsis thaliana | Hordeum vulgare thioredoxin 2 disulfide + NADPH + H+ | - |
? | |
| 1.8.1.9 | Hordeum vulgare thioredoxin 2 + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 2 disulfide + NADPH + H+ | - |
? | |
| 1.8.1.9 | Hordeum vulgare thioredoxin 2 mutant E86A + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 2 mutant E86A disulfide + NADPH + H+ | - |
? | |
| 1.8.1.9 | Hordeum vulgare thioredoxin 2 mutant G47P + NADP+ | - |
Arabidopsis thaliana | Hordeum vulgare thioredoxin 2 mutant G47P disulfide + NADPH + H+ | - |
? | |
| 1.8.1.9 | Hordeum vulgare thioredoxin 2 mutant G57P + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 2 mutant G57P disulfide + NADPH + H+ | - |
? | |
| 1.8.1.9 | Hordeum vulgare thioredoxin 2 mutant I51G + NADP+ | - |
Arabidopsis thaliana | Hordeum vulgare thioredoxin 2 mutant I51G disulfide + NADPH + H+ | - |
? | |
| 1.8.1.9 | Hordeum vulgare thioredoxin 2 mutant I51G + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 2 mutant I51G disulfide + NADPH + H+ | - |
? | |
| 1.8.1.9 | additional information | binding of NADP+ enhances the ability of Ntr2 to bind thioredoxin | Hordeum vulgare subsp. vulgare | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | NTR2 | - |
Hordeum vulgare subsp. vulgare |
| 1.8.1.9 | NtrB | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 1.8 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 2.6 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 3.9 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 4.2 | - |
Hordeum vulgare thioredoxin 2 | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 4.2 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 4.8 | - |
Arabidopsis thaliana thioredoxin 3 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 5 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 5 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 5.1 | - |
Hordeum vulgare thioredoxin 2 | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 5.3 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 5.9 | - |
Arabidopsis thaliana thioredoxin 3 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.8.1.9 | 7.3 | - |
Hordeum vulgare thioredoxin 2 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 7.9 | - |
Hordeum vulgare thioredoxin 2 mutant G57P | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 7.9 | - |
Hordeum vulgare thioredoxin 2 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 8 | - |
Hordeum vulgare thioredoxin 2 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 8.1 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 8.5 | - |
Hordeum vulgare thioredoxin 2 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.8.1.9 | 9.4 | - |
Escherichia coli thioredoxin | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 10.4 | - |
Hordeum vulgare thioredoxin 2 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 10.4 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 10.7 | - |
Arabidopsis thaliana thioredoxin 3 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 11 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A/M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 11.4 | - |
Hordeum vulgare thioredoxin 2 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 12.1 | - |
Hordeum vulgare thioredoxin 1 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 14.8 | - |
Arabidopsis thaliana thioredoxin 3 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 15.3 | - |
Arabidopsis thaliana thioredoxin 3 | mutant Delta42-47, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 15.9 | - |
Hordeum vulgare thioredoxin 2 mutant I51G | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | FAD | - |
Arabidopsis thaliana | |
| 1.8.1.9 | FAD | - |
Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | NADP+ | - |
Arabidopsis thaliana | |
| 1.8.1.9 | NADP+ | - |
Hordeum vulgare subsp. vulgare | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 63.1 | - |
Escherichia coli thioredoxin | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 140 | - |
Hordeum vulgare thioredoxin 2 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.8.1.9 | 228 | - |
Hordeum vulgare thioredoxin 2 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 282 | - |
Arabidopsis thaliana thioredoxin 3 | mutant Delta42-47, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 361 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A/M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 713 | - |
Hordeum vulgare thioredoxin 2 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 1030 | - |
Hordeum vulgare thioredoxin 2 mutant I51G | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 1050 | - |
Arabidopsis thaliana thioredoxin 3 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 1490 | - |
Hordeum vulgare thioredoxin 2 | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 1630 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 1710 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 2550 | - |
Arabidopsis thaliana thioredoxin 3 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 3300 | - |
Hordeum vulgare thioredoxin 2 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 4240 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 5340 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 5410 | - |
Hordeum vulgare thioredoxin 2 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 5490 | - |
Hordeum vulgare thioredoxin 2 mutant G57P | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 6000 | - |
Arabidopsis thaliana thioredoxin 3 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 6180 | - |
Hordeum vulgare thioredoxin 2 | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 6330 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 6800 | - |
Hordeum vulgare thioredoxin 2 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 7460 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 9230 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 9620 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 9840 | - |
Hordeum vulgare thioredoxin 1 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 10200 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1.8.1.9 | 12300 | - |
Arabidopsis thaliana thioredoxin 3 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana |
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