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Literature summary extracted from

  • Paukner, R.; Staudigl, P.; Choosri, W.; Haltrich, D.; Leitner, C.
    Expression, purification, and characterization of galactose oxidase of Fusarium sambucinum in E. coli (2015), Protein Expr. Purif., 108, 73-79 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.9 Tween 80 slight activation Fusarium sambucinum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.9 gene gaoA, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Fusarium sambucinum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.9 EDTA 56% inhibition Fusarium sambucinum
1.1.3.9 KCN complete inhibition Fusarium sambucinum
1.1.3.9 NaN3 complete inhibition Fusarium sambucinum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.9 additional information no or poor effects by Mg2+, K+, Na+, NH4+, Mn2+, and NaF Fusarium sambucinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.9 D-galactose + O2 Fusarium sambucinum
-
 D-galacto-hexodialdose + H2O2
-
 ?
1.1.3.9 D-galactose + O2 Fusarium sambucinum MA1886
-
 D-galacto-hexodialdose + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.9 Fusarium sambucinum A0A089QAB6 i.e. Gibberella pulicaris
-
1.1.3.9 Fusarium sambucinum MA1886 A0A089QAB6 i.e. Gibberella pulicaris
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.9 recombinant C-terminally His6-tagged enzyme 204fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Fusarium sambucinum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.9 159
-
 purified recombinant His-tagged enzyme, substrate D-galactose, pH 7.0, 30°C Fusarium sambucinum
1.1.3.9 226
-
 purified recombinant His-tagged enzyme, substrate 1-methyl-beta-D-galactopyranoside, pH 7.0, 30°C Fusarium sambucinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.9 1-methyl-beta-D-galactopyranoside + O2 high activity Fusarium sambucinum ? + H2O2
-
 ?
1.1.3.9 1-methyl-beta-D-galactopyranoside + O2 high activity Fusarium sambucinum MA1886 ? + H2O2
-
 ?
1.1.3.9 D-galactose + O2
-
 Fusarium sambucinum D-galacto-hexodialdose + H2O2
-
 ?
1.1.3.9 D-galactose + O2
-
 Fusarium sambucinum MA1886 D-galacto-hexodialdose + H2O2
-
 ?
1.1.3.9 lactose + O2
-
 Fusarium sambucinum ? + H2O2
-
 ?
1.1.3.9 lactose + O2
-
 Fusarium sambucinum MA1886 ? + H2O2
-
 ?
1.1.3.9 melibiose + O2 high catalytic efficiency Fusarium sambucinum ? + H2O2
-
 ?
1.1.3.9 melibiose + O2 high catalytic efficiency Fusarium sambucinum MA1886 ? + H2O2
-
 ?
1.1.3.9 additional information standard ABTS assay. The enzyme is highly specific for molecular oxygen as an electron acceptor, and shows no appreciable activity with a range of alternative acceptors investigated, no activity with ABTS cation radical, ferrocenium ion, 1,4-benzoquinone, 2,6-dichloro-indophenol (DCIP), ferricyanide, guaiacol radical, 2,6-dimethoxyphenol radical, caffeic acid radical, p-coumaric acid radical, ferulic acid radical, sinapic acid radical, thioflavin T, 2-(4'-methylaminophenyl)benzothiazole and 1,10-diethyl-2,20-carbocyanine iodide Fusarium sambucinum ?
-
 ?
1.1.3.9 additional information standard ABTS assay. The enzyme is highly specific for molecular oxygen as an electron acceptor, and shows no appreciable activity with a range of alternative acceptors investigated, no activity with ABTS cation radical, ferrocenium ion, 1,4-benzoquinone, 2,6-dichloro-indophenol (DCIP), ferricyanide, guaiacol radical, 2,6-dimethoxyphenol radical, caffeic acid radical, p-coumaric acid radical, ferulic acid radical, sinapic acid radical, thioflavin T, 2-(4'-methylaminophenyl)benzothiazole and 1,10-diethyl-2,20-carbocyanine iodide Fusarium sambucinum MA1886 ?
-
 ?
1.1.3.9 raffinose + O2
-
 Fusarium sambucinum 6''-aldehydoraffinose + 6''-carboxyraffinose + H2O2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.9 ? x * 68500, recombinant His6-tagged enzyme, SDS-PAGE Fusarium sambucinum
1.1.3.9 More three-dimensional structure modeling based on the structure of GalOx from Fusarium graminearum Fusarium sambucinum

Synonyms

EC Number Synonyms Comment Organism
1.1.3.9 GalOx
-
 Fusarium sambucinum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.9 35
-
 recombinant His-tagged enzyme Fusarium sambucinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.9 40 60 the purified recombinant His-tagged enzyme shows half-lifes of 11.2 min, 5.3 min, and 2.7 min for incubation at 40°C, 50°C, and 60°C, respectively Fusarium sambucinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.9 6 7.5 recombinant His-tagged enzyme Fusarium sambucinum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.9 5.5 9 activity range, more than 95% activity in the range of pH 6.0-7.5, profile overview. Inactive at pH 5.0 and below Fusarium sambucinum

General Information

EC Number General Information Comment Organism
1.1.3.9 additional information three-dimensional structure modeling based on the structure of GalOx from Fusarium graminearum Fusarium sambucinum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.9 2700
-
 melibiose recombinant His-tagged enzyme, pH 7.0, 30°C Fusarium sambucinum