BRENDA - Enzyme Database

High yield purification of a tagged cytoplasmic [NiFe]-hydrogenase and a catalytically-active nickel-free intermediate form

Chandrayan, S.K.; Wu, C.H.; McTernan, P.M.; Adams, M.W.; Protein Expr. Purif. 107, 90-94 (2015)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.12.99.6
cytoplasm
-
Pyrococcus furiosus
5737
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.99.6
H2 + NADPH
Pyrococcus furiosus
-
NADP + H+
-
-
?
1.12.99.6
H2 + NADPH
Pyrococcus furiosus MW0430
-
NADP + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.12.99.6
Pyrococcus furiosus
-
-
-
1.12.99.6
Pyrococcus furiosus MW0430
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.12.99.6
Ni-NTA column chromatography
Pyrococcus furiosus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.99.6
H2 + NADPH
-
743732
Pyrococcus furiosus
NADP + H+
-
-
-
?
1.12.99.6
H2 + NADPH
-
743732
Pyrococcus furiosus MW0430
NADP + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.12.99.6
heterotetramer
-
Pyrococcus furiosus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.12.99.6
FAD
-
Pyrococcus furiosus
1.12.99.6
[2Fe-2S]-center
-
Pyrococcus furiosus
1.12.99.6
[4Fe-4S]-center
-
Pyrococcus furiosus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.12.99.6
FAD
-
Pyrococcus furiosus
1.12.99.6
[2Fe-2S]-center
-
Pyrococcus furiosus
1.12.99.6
[4Fe-4S]-center
-
Pyrococcus furiosus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.12.99.6
cytoplasm
-
Pyrococcus furiosus
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.99.6
H2 + NADPH
Pyrococcus furiosus
-
NADP + H+
-
-
?
1.12.99.6
H2 + NADPH
Pyrococcus furiosus MW0430
-
NADP + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.12.99.6
Ni-NTA column chromatography
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.99.6
H2 + NADPH
-
743732
Pyrococcus furiosus
NADP + H+
-
-
-
?
1.12.99.6
H2 + NADPH
-
743732
Pyrococcus furiosus MW0430
NADP + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.12.99.6
heterotetramer
-
Pyrococcus furiosus