EC Number | Cloned (Comment) | Organism |
---|---|---|
1.11.1.19 | expressed in Escherichia coli BL21(DE3)pLyS cells | Auricularia auricula-judae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.19 | H2O2 | - |
Auricularia auricula-judae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.19 | 0.03 | - |
Reactive Black 5 | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
1.11.1.19 | 0.12 | - |
Reactive Blue 19 | wild type enzyme, at pH 3.5 and 30°C | Auricularia auricula-judae | |
1.11.1.19 | 0.283 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
1.11.1.19 | 1.32 | - |
2,6-dimethoxyphenol | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.19 | Auricularia auricula-judae | I2DBY1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.19 | Q Sepharose column chromatography, Mono Q column chromatography, and Mono P column chromatography | Auricularia auricula-judae |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.11.1.19 | with oxidized glutathione (1.5 mM), hemin (0.03 mM) and urea (0.8 M) | Auricularia auricula-judae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.19 | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Auricularia auricula-judae | ? | - |
? | |
1.11.1.19 | 2,6-dimethoxyphenol + H2O2 + H+ | - |
Auricularia auricula-judae | ? | - |
? | |
1.11.1.19 | Reactive Black 5 + H2O2 + H+ | - |
Auricularia auricula-judae | ? | - |
? | |
1.11.1.19 | Reactive Blue 19 + H2O2 + H+ | - |
Auricularia auricula-judae | ? | - |
? | |
1.11.1.19 | veratryl alcohol + H2O2 + H+ | very weak substrate | Auricularia auricula-judae | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.11.1.19 | ? | x * 47000, calculated from amino acid sequence | Auricularia auricula-judae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.11.1.19 | dye-decolorizing peroxidase | - |
Auricularia auricula-judae |
1.11.1.19 | DyP | - |
Auricularia auricula-judae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.19 | 30 | 65 | the wild type enzyme remains stable after 24 h at 30-55°C and shows 50% activity after 24 h at 65.5°C | Auricularia auricula-judae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.19 | 10.6 | - |
Reactive Black 5 | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
1.11.1.19 | 420 | - |
2,6-dimethoxyphenol | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
1.11.1.19 | 422 | - |
Reactive Blue 19 | wild type enzyme, at pH 3.5 and 30°C | Auricularia auricula-judae | |
1.11.1.19 | 654 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.19 | 4 | 9 | the enzyme shows more than 80% activity after 24 h at pH 4.0-9.0 | Auricularia auricula-judae |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.11.1.19 | Auricularia auricula-judae | isoelectric focusing | - |
4.4 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.19 | 0.11 | - |
veratryl alcohol | wild type enzyme, at pH 2.5 and 30°C | Auricularia auricula-judae | |
1.11.1.19 | 320 | - |
2,6-dimethoxyphenol | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
1.11.1.19 | 400 | - |
Reactive Black 5 | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
1.11.1.19 | 2300 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
1.11.1.19 | 3600 | - |
Reactive Blue 19 | wild type enzyme, at pH 3.5 and 30°C | Auricularia auricula-judae |