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Literature summary extracted from

  • Li, X.J.; Zheng, R.C.; Wu, Z.M.; Ding, X.; Zheng, Y.G.
    Thermophilic esterase from Thermomyces lanuginosus molecular cloning, functional expression and biochemical characterization (2014), Protein Expr. Purif., 101, 1-7 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.1 synthesis enzyme shows high enantioselectivity in the kinetic resolution of 2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester, which produce a valuable chiral intermediate-(3S)-2-carboxyethyl-3-cyano-5-methylhexanoic acid for the drug Pregabalin Thermomyces lanuginosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expression in Escherichia coli Thermomyces lanuginosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 Ba2+
-
Thermomyces lanuginosus
3.1.1.1 Ca2+
-
Thermomyces lanuginosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Thermomyces lanuginosus W8DVW0
-
-
3.1.1.1 Thermomyces lanuginosus DSM 10635 W8DVW0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester + H2O
-
Thermomyces lanuginosus (3S)-2-carboxyethyl-3-cyano-5-methylhexanoic acid + ethanol
-
?
3.1.1.1 4-nitrophenyl acetate + H2O about 55% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus 4-nitrophenol + acetate
-
?
3.1.1.1 4-nitrophenyl acetate + H2O about 55% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus DSM 10635 4-nitrophenol + acetate
-
?
3.1.1.1 4-nitrophenyl butanoate + H2O best substrate Thermomyces lanuginosus 4-nitrophenol + butanoate
-
?
3.1.1.1 4-nitrophenyl decanoate + H2O about 10% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus 4-nitrophenol + decanoate
-
?
3.1.1.1 4-nitrophenyl decanoate + H2O about 10% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus DSM 10635 4-nitrophenol + decanoate
-
?
3.1.1.1 4-nitrophenyl hexanoate + H2O about 65% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus 4-nitrophenol + hexanoate
-
?
3.1.1.1 4-nitrophenyl hexanoate + H2O about 65% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus DSM 10635 4-nitrophenol + hexanoate
-
?
3.1.1.1 4-nitrophenyl octanoate + H2O about 30% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus 4-nitrophenol + octanoate
-
?
3.1.1.1 4-nitrophenyl octanoate + H2O about 30% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus DSM 10635 4-nitrophenol + octanoate
-
?
3.1.1.1 additional information no substrate: 4-nitrophenyl palmitate Thermomyces lanuginosus ?
-
?
3.1.1.1 additional information no substrate: 4-nitrophenyl palmitate Thermomyces lanuginosus DSM 10635 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 34500, calculated, x * 35000, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 60
-
-
Thermomyces lanuginosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 65
-
80% of maximum activtiy Thermomyces lanuginosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 60
-
8 h, 50% resiudal activity Thermomyces lanuginosus
3.1.1.1 70
-
60 min, 53% residual activity Thermomyces lanuginosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 8.5
-
-
Thermomyces lanuginosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 7.5 9 low activity below or above Thermomyces lanuginosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.1 7 9 12 h, 80% residual activity Thermomyces lanuginosus