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Literature summary extracted from

  • Svetlikova, Z.; Barath, P.; Jackson, M.; Kordulakova, J.; Mikusova, K.
    Purification and characterization of the acyltransferase involved in biosynthesis of the major mycobacterial cell envelope glycolipid-monoacylated phosphatidylinositol dimannoside (2014), Protein Expr. Purif., 100, 33-39 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.265 expressed in Escherichia coli BL21(DE3)pLysS, Rosetta-gami (DE3)pLysS, and C41(DE3)cells Mycolicibacterium smegmatis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.265 cell envelope
-
 Mycolicibacterium smegmatis 30313
-
2.3.1.265 cytosol
-
 Mycolicibacterium smegmatis 5829
-
2.3.1.265 membrane
-
 Mycolicibacterium smegmatis 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.265 Mycolicibacterium smegmatis A0QWG5
-
-
2.3.1.265 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 A0QWG5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.265 TALON Co2+ affinity resin column chromatography, and Superdex 200 gel filtration Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.265 palmitoyl-CoA + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol preferred substrate Mycolicibacterium smegmatis CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 ?
2.3.1.265 palmitoyl-CoA + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol preferred substrate Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 ?
2.3.1.265 palmitoyl-CoA + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 Mycolicibacterium smegmatis CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
 ?
2.3.1.265 palmitoyl-CoA + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.265 ? x * 34000, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.265 MSMEG 2934 protein
-
 Mycolicibacterium smegmatis