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Literature summary extracted from

  • Wolski, P.; Dana, C.; Clark, D.; Blanch, H.
    Engineering ionic liquid-tolerant cellulases for biofuels production (2016), Protein Eng. Des. Sel., 29, 117-122 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4
-
Rasamsonia emersonii

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 A3V/A6Q/T7K/A8P/N10T/E18K/P22D/P58T/Y60L/N157A/D181N/E183Q mutant is more active and stable than wild-type Cel7A or Trichoderma reesei Cel7A in aqueous ionic liquids solutions, i.e. up to 43% (w/w) 1,3-dimethylimdazolium dimethylphosphate and 20% (w/w) 1-ethyl-3-methylimidazolium acetate Rasamsonia emersonii
3.2.1.4 A6L/A8E/N10V/P58E/T59S/Y60L/L73V/G80A/V84I/S87N/S89D/K92T/L105V/L108M/Q109E/N220T/V222F/S301K/I308V/S311G/N312K/Q316N/P317S/N318E/D320T/I321W/T325G/T438N/G439P/T440P/P441G/S442G/H471M mutant is more active and stable than wild-type Cel7A or Trichoderma reesei Cel7A in aqueous–ionic liquids solutions, i.e. up to 43% (w/w) 1,3-dimethylimdazolium dimethylphosphate and 20% (w/w) 1-ethyl-3-methylimidazolium acetate. Increase in melting temperature of 1.9-3.9°C compared to wild-type Rasamsonia emersonii
3.2.1.4 additional information post-translational modification of the N-terminal glutamine residue to diglutamate via glutaminyl cyclase enhances the stability of Cel7A and variants Rasamsonia emersonii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Rasamsonia emersonii Q8TFL9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 4-methylumbelliferyl beta-D-lactoside + H2O
-
Rasamsonia emersonii 4-methylumbelliferone + D-lactose
-
?
3.2.1.4 avicel + H2O
-
Rasamsonia emersonii ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cel7A
-
Rasamsonia emersonii