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Literature summary extracted from
- Advanced evolutionary molecular engineering to produce thermostable cellulase by using a small but efficient library (2013), Protein Eng. Des. Sel., 26, 73-79 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | expression in Saccharomyces cerevisiae | Phanerodontia chrysosporium |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | A241E | improved thermal stability | Phanerodontia chrysosporium |
| 3.2.1.4 | F206Y | improved thermal stability | Phanerodontia chrysosporium |
| 3.2.1.4 | M259I | improved thermal stability | Phanerodontia chrysosporium |
| 3.2.1.4 | M259L | improved thermal stability | Phanerodontia chrysosporium |
| 3.2.1.4 | N125K | improved thermal stability | Phanerodontia chrysosporium |
| 3.2.1.4 | Q204K | improved thermal stability | Phanerodontia chrysosporium |
| 3.2.1.4 | Q307R/A309S | improved thermal stability | Phanerodontia chrysosporium |
| 3.2.1.4 | Q381S/F382L | improved thermal stability | Phanerodontia chrysosporium |
| 3.2.1.4 | S183T | improved thermal stability | Phanerodontia chrysosporium |
| 3.2.1.4 | S22P | thermostability similar to wild-type | Phanerodontia chrysosporium |
| 3.2.1.4 | T112S | improved thermal stability | Phanerodontia chrysosporium |
| 3.2.1.4 | T130I/S134Q | improved thermal stability | Phanerodontia chrysosporium |
| 3.2.1.4 | T130I/S134Q/M259I/A241E/S183T/Q307R/A309S | temperature required to reduce the initial activity by 50% within 120 min is increased by 4.4 degrees compared to wild-type | Phanerodontia chrysosporium |
| 3.2.1.4 | T130I/S134Q/M259I/A241E/S183T/Q307R/A309S/T112S/N125K/Q204K/F206Y/I365 | temperature required to reduce the initial activity by 50% within 120 min is increased by 5.4 degrees compared to wild-type, specific activity is the same as wild-type | Phanerodontia chrysosporium |
| 3.2.1.4 | T342HI365V | improved thermal stability | Phanerodontia chrysosporium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | Phanerodontia chrysosporium | Q02321 | - |
- |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | CBHII | - |
Phanerodontia chrysosporium |
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Release 2023.1 (January 2023)
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