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Literature summary extracted from

  • Long, Y.; Yang, S.; Xie, Z.; Cheng, L.
    Cloning, expression, and characterization of catechol 1,2-dioxygenase from a phenol-degrading Candida tropicalis JH8 strain (2016), Prep. Biochem. Biotechnol., 46, 673-678 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.1 expressed in Escherichia coli Candida tropicalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.0092
-
catechol at pH 7.5 and 30°C Candida tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.1 catechol + O2 Candida tropicalis
-
cis,cis-muconate
-
?
1.13.11.1 catechol + O2 Candida tropicalis JH8
-
cis,cis-muconate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Candida tropicalis
-
-
-
1.13.11.1 Candida tropicalis JH8
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 catechol + O2
-
Candida tropicalis cis,cis-muconate
-
?
1.13.11.1 catechol + O2
-
Candida tropicalis JH8 cis,cis-muconate
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 homodimer 2 * 32000, SDS-PAGE Candida tropicalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 30
-
-
Candida tropicalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7.5
-
-
Candida tropicalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.1 7 9 recombinant enzyme is unstable below pH 5.0 and stable from pH 7.0 to 9.0 Candida tropicalis